S9636

Superoxide Dismutase from human erythrocytes

essentially salt-free, lyophilized powder, ≥2,500 units/mg protein

• Szinonimák: SOD, Superoxide: superoxide oxidoreductase
• CAS-szám: 9054-89-1
• Enzyme Commission szám: 1.15.1.1 (BRENDA, IUBMB)
• EC-szám: 232-943-0
• MDL-szám: MFCD00132404
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: human erythrocytes
• Minőségi szint: 400
• Teszt: >80% protein (biuret)
• Forma: essentially salt-free, lyophilized powder
• specifikus aktivitás: ≥2,500 units/mg protein
• molekulatömeg: 32.0 kDa
• összetétel: Protein, ≥80% biuret
• gyártó/kereskedő neve: Sigma-Aldrich
• technika/technikák: activity assay: suitable
• szín: white to off-white
• pH-tartomány: 7.6—10.5
• pH: 7.8
• alkalmasság: suitable for molecular biology
• UniProt elérési szám: P00441; P04179; P08294
• alkalmazás(ok): life science and biopharma
• tárolási hőmérséklet: −20°C
• Géninformáció: human ... SOD1(6647) , SOD2(6648) , SOD3(6649)

Leírás

Általános leírás

Superoxide dismutases (SOD) are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. Three forms of SOD exist, based on the metal ions in the active site. These are Cu²⁺/Zn²⁺, Mn²⁺ and Fe²⁺ containing SOD. In vertebrate organisms, Cu/Zn-SOD is located in the cytoplasm as well as the mitochondrial intermembrane space, whereas Mn-SOD is located at the mitochondrial matrix space in prokaryotes. Fe-SOD is also found in prokaryotes and higher plants. Human erythrocyte SOD is a non-covalently bound homodimeric protein with two 16.3 kDa subunits containing 153 amino acids. Each dimer consists of two Cu²⁺ atoms and two Zn²⁺ atoms.

Alkalmazás

Superoxide Dismutase from human erythrocytes has been used:

• to test its effect on human neutrophils in reactive oxygen species (ROS) measurement studies involving Pseudomonas aeruginosa infection
• as an antioxidant to test its effect on ROS generation induced by atmospheric-pressure plasma jet (APPJ) in red blood cell (RBC) homogenates using optical spectroscopy studies
• to test its attenuating effect on hemoglobin (Hb)-induced nuclear factor-kappa B (NF- κB) and hypoxia-inducible factor (HIF) activity in human dermal microvascular endothelial cells (HMECs-1)
• as a reference antioxidant protein to examine its expression in human intestinal Caco-2 cells following treatment with dietary flavonoids
• in combination with catalase to promote cell differentiation in vitro

Biokémiai/fiziológiai hatások

Mutations in the SOD1 gene are implicated in Amyotrophic lateral sclerosis (ALS).

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Egység definíció

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Analízis megjegyzés

For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)