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Merck

RAB0026

Sigma-Aldrich

Human BDNF ELISA Kit

for serum, plasma, cell culture supernatant and urine

Szinonimák:

BDNF, Brain-derived neurotrophic factor

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41116158
NACRES:
NA.32
Az árazás és az elérhetőség jelenleg nem elérhető

faj reaktivitás

human

kiszerelés

kit of 96 wells (12 strips x 8 wells)

technika/technikák

ELISA: suitable
capture ELISA: suitable

bemenet

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.066-16 ng/mL

észlelési módszer

colorimetric

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

Géninformáció

human ... BDNF(627)

Általános leírás

The Human BDNF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, cell culture supernatants and urine.
The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1.[1] BDNF is a member of the neurotrophin family of growth factors.[2] The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling.[3]

Immunogén

Recombinant Human BDNF

Alkalmazás

For research use only. Not for use in diagnostic procedures.
Human BDNF ELISA kit has been used to determine the effect of bone marrow mesenchymal stem cell (MSC) transplantation on BDNF protein expression in the striatum of Tourette syndrome (TS) rats.[4] It has also been used to determine the plasma BDNF levels in GD1 (Gaucher′s disease type 1) patients and control population.[5]
Human BDNF ELISA Kit has been used:
  • to measure the brain-derived neurotrophic factor (BDNF) protein expression[6]
  • to determine BDNF level in the slice culture medium and the extracts of organotypic brain slice cultures (OBSCs)[7]
  • to determine BDNF level in plasma[8]

Biokémiai/fiziológiai hatások

Brain derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. Hence, variation in the BDNF gene expression alters these neurological functions.[9] BDNF also plays a vital role in vascular function and participates in angiogenesis via the specific receptor tropomyosin-related kinase B (TrkB).[10] It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.[3]

Egyéb megjegyzések

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Leírás
Biztonsági adatlap

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)Biztonsági adatlap

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)Biztonsági adatlap

  • RABSTOP3ELISA Stop Solution (Item I)Biztonsági adatlap

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)Biztonsági adatlap

  • RABWASH420X Wash Buffer (Item B)Biztonsági adatlap

Piktogramok

Corrosion

Figyelmeztetés

Warning

Figyelmeztető mondatok

Óvintézkedésre vonatkozó mondatok

Veszélyességi osztályok

Met. Corr. 1

Tárolási osztály kódja

8A - Combustible corrosive hazardous materials


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Oxidative Stress-Tolerant Stem Cells from Human Exfoliated Deciduous Teeth Decrease Hydrogen Peroxide-Induced Damage in Organotypic Brain Slice Cultures from Adult Mice
Xiao L, et al.
International Journal of Molecular Sciences, 20(8), 1858-1858 (2019)
Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome
Liu X, et al.
Experimental and Therapeutic Medicine, 11(4), 1211-1216 (2016)
Xiumei Liu et al.
Experimental and therapeutic medicine, 11(4), 1211-1216 (2016-04-14)
The aim of the present study was to investigate the effect of bone marrow mesenchymal stem cell (MSC) transplantation on brain-derived neurotrophic factor (BDNF) expression in the striatum of Tourette syndrome (TS) rats. In addition, the possible mechanism of MSC
Shervin Pejhan et al.
Frontiers in cell and developmental biology, 8, 763-763 (2020-09-26)
Rett Syndrome (RTT) is a rare and progressive neurodevelopmental disorder that is caused by de novo mutations in the X-linked Methyl CpG binding protein 2 (MECP2) gene and is subjected to X-chromosome inactivation. RTT is commonly associated with neurological regression
Zuzana Paduchová et al.
Nutrients, 13(4) (2021-04-04)
In the DEPOXIN project, we have found that a high ratio of omega-6/omega-3 fatty acids (FA) is associated with worsening of depressive symptoms in children and adolescents with depressive disorder (DD) and that the 12-week omega-3 FA supplementation modulates DD

Questions

  1. Could you please confirm if this kit is capable of recognizing both free and bound BDNF?

    1 answer
    1. Epitope mapping has not been conducted, so the exact binding site is unknown. However, the immunogen is located at aa128-247 (Accession# P23560).

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