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P6403

Sigma-Aldrich

Poly-D-lysine hydrobromide

suitable for cell culture, Mol wt 4,000-15,000

Szinonimák:

D-Lysine homopolymer hydrobromide

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About This Item

Lineáris képlet:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS-szám:
27964-99-4
MDL-szám:
MFCD00131934
UNSPSC kód:
12352209
PubChem Substance ID:
24898763
NACRES:
NA.26

product name

Poly-D-lysine hydrobromide, mol wt 4,000-15,000

form

powder or solid

Minőségi szint

200

molekulatömeg

4,000-15,000

technika/technikák

cell culture | mammalian: suitable

szín

white to light yellow

tárolási hőmérséklet

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

Nemzetközi kémiai azonosító kulcs

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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Alkalmazás

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biokémiai/fiziológiai hatások

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Komponensek

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Vigyázat

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Elkészítési megjegyzés

This product has a molecular weight of 4,000-15,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Allyson E Sgro et al.
Journal of neuroscience methods, 198(2), 230-235 (2011-04-26)
Generating microislands of culture substrate on coverslips by spray application of poly-d lysine is a commonly used method for culturing isolated neurons that form self (autaptic) synapses. This preparation has multiple advantages for studying synaptic transmission in isolation; however, generating
Munehiro Yamaguchi et al.
Langmuir : the ACS journal of surfaces and colloids, 27(20), 12521-12532 (2011-09-09)
Micropatterning techniques have become increasingly important in cellular biology. Cell patterning is achieved by various methods. Photolithography is one of the most popular methods, and several light sources (e.g., excimer lasers and mercury lamps) are used for that purpose. Vacuum
Kyungtae Kang et al.
Biomaterials, 32(27), 6374-6380 (2011-06-10)
Chemical surface modification of neuron-surface interfaces is essential for the development of biologically active and functional neural interfaces. Different types of surface modification schemes are required to derivatize either electrode or insulator surfaces, which limits the surface chemistry based neural
Zhou Xu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(10), 3426-3435 (2011-06-24)
Misfolding of the prion protein (PrP) is the central feature of prion diseases. The conversion of the normal α-helical PrP(C) into a pathological β-enriched PrP(Sc) constitutes an early event in the infectious process. Several hypotheses, involving different regions of the
Yong Hee Kim et al.
Journal of neuroscience methods, 202(1), 38-44 (2011-09-13)
We have prepared the poly-d-lysine (PDL) bound surfaces for neuron cell culture by covalent binding between the poly-d-Lysine and substrates and investigated neuronal cell adhesion properties and cell growth morphology. The number of neuronal cell and the number of neurite
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