Összes fotó(3)
Fontos dokumentumok
N5415
Nerve Growth Factor-ß human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Szinonimák:
β-NGF, BNGF, NGF-β, NGF-beta
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352202
NACRES:
NA.77
Javasolt termékek
product name
Nerve Growth Factor-ß human, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
biológiai forrás
human
Minőségi szint
rekombináns
expressed in HEK 293 cells
Teszt
≥95% (SDS-PAGE)
form
lyophilized powder
hatékonyság
≤16 ng/mL ED50/EC50
molekulatömeg
protein calculated mol wt 13.5 kDa (Monomer)
kiszerelés
pkg of 10 μg
pkg of 100 μg
technika/technikák
cell culture | mammalian: suitable
szennyeződések
≤1 EU/μg Endotoxin (β-NGF)
UniProt elérési szám
tárolási hőmérséklet
−20°C
Géninformáció
human ... NGF(4803)
Általános leírás
NGF-β gene is mapped to human chromosome 1p13.2.
Recombinant human β-NGF is expressed in human 293 cells as a homodimer (non-disulfide bonded) of 120 amino acids, with a calculated molecular weight of 13.5 kDa (monomer). Nerve growth factor β (β-NGF) is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The production of natural β-NGF occurs in epithelial cells, and the pituitary gland.
Biokémiai/fiziológiai hatások
A mutation in the NGF-β gene is implicated in the loss of pain perception.
Nerve growth factor β (β-NGF) is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.
Vigyázat
The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at -20 °C.
Fizikai forma
This product is lyophilized from PBS.
Elkészítési megjegyzés
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile DDW containing 0.1% endotoxin-free recombinant human serum albumin, to a final concentration of 100 μg/ml.
Tárolás és stabilitás
Store product at –20 °C. The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at –20 °C.
Analízis megjegyzés
The specific activity is determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Jogi nyilatkozat
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az ügyfelek ezeket is megtekintették
S Soinila et al.
Neuroscience research, 6(2), 123-130 (1988-12-01)
Nerve growth factor (NGF)-like immunoreactivity was demonstrated in the rat pituitary intermediate lobe by indirect immunofluorescence method using antisera specific to beta-NGF isolated from adult male mouse submaxillary salivary gland. Co-culture of frozen or fresh intermediate lobes with newborn rat
A Rosenthal et al.
Handbook of experimental pharmacology, 220, 497-512 (2014-03-29)
The neurotrophin family is comprised of the structurally related secreted proteins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophine-4 (NT-4). They bind and activate the tyrosine kinase receptors Trk A, B, and C in a ligand-specific
Katherine M Nielsen et al.
Molecular and cellular neurosciences, 25(4), 629-641 (2004-04-15)
Developing neurons encounter a panoply of extracellular signals as they differentiate. A major goal is to identify these extrinsic cues and define the mechanisms by which neurons simultaneously integrate stimulation by multiple factors yet initiate one specific biological response. Factors
Yousong Su et al.
Progress in neuro-psychopharmacology & biological psychiatry, 104, 110015-110015 (2020-06-23)
The neurodevelopmental hypothesis of schizophrenia has been widely accepted. In light of our previous microarray data, two neurodevelopment-related genes were focused on inclduing the N-acylsphingosine amidohydrolase 1 gene (ASAH1) and the nerve growth factor gene (NGF). The evidence that ASAH1
Tissue localization of nerve growth factor and nerve growth factor receptors.
M Bothwell
Current topics in microbiology and immunology, 165, 55-70 (1991-01-01)
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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