Considering the significant quenching of fluorescence by manganese (Mn), it is not suitable for the assay. Instead, the use of MgCl2 (up to 1 mM) is suggested as it offers less interference. Additionally, DTT can be used up to 1 mM.
Összes fotó(2)
Fontos dokumentumok
MAK168
Pyrophosphate Assay Kit
sufficient for 200 fluorometric tests (Blue fluorescence)
Szinonimák:
High-Sensitivity Pyrophosphate Assay Kit
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
1 KIT
123 000,00 Ft
123 000,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben
Összes fotó(2)
Méret kiválasztása
Nézet módosítása
1 KIT
123 000,00 Ft
About This Item
123 000,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben
Javasolt termékek
használat
sufficient for 200 fluorometric tests (Blue fluorescence)
alkalmazás(ok)
cosmetics
food and beverages
észlelési módszer
fluorometric
tárolási hőmérséklet
−20°C
Általános leírás
Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis,[1] DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. It is hydrolyzed by inorganic pyrophosphatase. PPi is present in the blood and in the extracellular matrix of tissues.[1]
Alkalmasság
The Pyrophosphate Assay Kit provides a simple and direct microplate assay procedure for measuring pyrophosphate in a variety of samples.
Elv
The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =316/λem = 456 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Dam. 1
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 3
Lobbanási pont (F)
188.6 °F
Lobbanási pont (C)
87 °C
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Az ügyfelek ezeket is megtekintették
Peiyi Zheng et al.
Journal of molecular biology, 431(4), 764-776 (2019-01-18)
Phosphopantothenoylcysteine (PPC) synthetase (PPCS) catalyzes nucleoside triphosphate-dependent condensation reaction between 4'-phosphopantothenate (PPA) and l-cysteine to form PPC in CoA biosynthesis. The catalytic mechanism of PPCS has not been resolved yet. Coenzyme A biosynthesis protein 2 (Cab2) possesses activity of PPCS
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.
Kottur J and Deepak T N
Nucleic Acids Research (2018)
Tyler M Weaver et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25494-25504 (2020-10-02)
During DNA replication, replicative DNA polymerases may encounter DNA lesions, which can stall replication forks. One way to prevent replication fork stalling is through the recruitment of specialized translesion synthesis (TLS) polymerases that have evolved to incorporate nucleotides opposite DNA
Priyanka Kushwaha et al.
Journal of cellular physiology, 235(10), 6673-6683 (2020-01-28)
The activation of the Wnt/β-catenin signaling pathway is critical for skeletal development but surprisingly little is known about the requirements for the specific frizzled (Fzd) receptors that recognize Wnt ligands. To define the contributions of individual Fzd proteins to osteoblast
Jithesh Kottur et al.
Nucleic acids research, 46(12), 5875-5885 (2018-06-01)
DNA synthesis by DNA polymerases (dPols) is central to duplication and maintenance of the genome in all living organisms. dPols catalyze the formation of a phosphodiester bond between the incoming deoxynucleoside triphosphate and the terminal primer nucleotide with the release
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Any challenges encountered with decreases in fluorescence due to buffer components in the assays, such as manganese (1 mM), DTT (5 mM), and the combination of manganese with a reducing agent (both BME and DTT)? It seems that the signal is significantly reduced, if not entirely abolished, when these components are included in the buffer, even with substantial amounts of the PPi standard.
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