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M9309

Sigma-Aldrich

McCoy′s 5A Medium

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Szinonimák:

5A basal medium

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About This Item

MDL-szám:
MFCD00217560
UNSPSC kód:
12352207
NACRES:
NA.71

product name

McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Minőségi szint

300

sterilitás

sterile-filtered

form

liquid

technika/technikák

cell culture | mammalian: suitable

szennyeződések

endotoxin, tested

alkatrészek

NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

Alkalmazás

McCoy′s 5A Medium has been used:
  • to culture HCT-116?cells
  • to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
  • to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies

Biokémiai/fiziológiai hatások

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

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kiegészítés

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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