HT29 Cell Line human

91072201, human colon, Epithelial

• Szinonimák: HT29 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• biológiai forrás: human colon
• növekedési mód: Adherent
• kariotípus: 2n = 46, hypertriploid
• morfológia: Epithelial
• termékek: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human Caucasian colon adenocarcinoma

Sejtvonal leírása

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

Alkalmazás

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

DNS profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

Táptalaj

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

Egyéb megjegyzések

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