Összes fotó(1)
Fontos dokumentumok
M7905
Monoclonal Anti-Myosin Light Chain Kinase antibody produced in mouse
clone K36, ascites fluid
Szinonimák:
Anti-KRP, Anti-MLCK, Anti-MLCK1, Anti-MLCK108, Anti-MLCK210, Anti-MMIHS, Anti-MMIHS1, Anti-MSTP083, Anti-MYLK1, Anti-smMLCK
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
konjugátum:
unconjugated
application:
ARR
IP
WB
IP
WB
klón:
K36, monoclonal
faj reaktivitás:
avian, mammals
citations:
68
technika/technikák:
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:10,000
microarray: suitable
western blot: 1:10,000
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
konjugátum
unconjugated
antitest forma
ascites fluid
antitest terméktípus
primary antibodies
klón
K36, monoclonal
tartalmaz
15 mM sodium azide
faj reaktivitás
avian, mammals
technika/technikák
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:10,000
izotípus
IgG2b
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... MYLK(4638)
Related Categories
Általános leírás
Monoclonal Anti-Myosin Light Chain Kinase (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin light chain kinase (MLCK) is a calcium/calmodulin-dependent enzyme (approx.160 kD). It is expressed in skeletal, cardiac, smooth and mammalian non-muscle cells. MLCK is encoded by the MYLK1 gene. This gene encodes telokin, non-muscle and the smooth muscle isoforms of MLCK. MLCK gene is mapped to the human chromosome location 3q21.1.
Egyediség
In immunoblotting, the antibody recognizes the myosin light chain kinase of smooth muscle from various sources including chicken gizzard, turkey gizzard, and pig stomach as well as the myosin light chain kinase of non-muscle cells such as cultured fibroblasts.
Immunogén
chicken gizzard myosin light chain kinase.
Alkalmazás
Monoclonal anti-myosin light chain kinase antibody has been used in:
- immunohistochemistry
- immunoblot (diluted 1:10,000) analysis
- flow cytometry
Biokémiai/fiziológiai hatások
Myosin Light Chain Kinase (MLCK) has a pivotal role in phosphorylating myosin regulatory light chains and hence facilitates its interaction with actin filaments for smooth muscle contraction. Monoclonal anti-myosin light chain kinase antibody can be used for studying the function of the enzyme and its interactions with other cell components. It can also be used in microarray and immunoprecipitation. Mouse anti-myosin light chain kinase antibody reacts specifically with myosin light chain kinase present in smooth muscle of chicken gizzard, turkey gizzard, and pig stomach. This product also shows reactivity for myosin light chain kinase of non-muscle cells like cultured fibroblasts.
The catalytic subunit of cardiac and skeletal muscle myosin light chain kinase is in the range of 80-95 kDa. Proteolysis of the myosin light chain kinase from chicken gizzard produces a 64 kDa fragment that neither binds to Ca2+/calmodulin nor exhibits catalytic activity and a 61 kDa peptide that is active in the absence of Ca2+/calmodulin.
Fizikai forma
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Tárolás és stabilitás
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Az ügyfelek ezeket is megtekintették
Ze-Yan Yu et al.
PloS one, 9(6), e99024-e99024 (2014-06-12)
To determine the mechanisms by which the α1A-adrenergic receptor (AR) regulates cardiac contractility. We reported previously that transgenic mice with cardiac-restricted α1A-AR overexpression (α1A-TG) exhibit enhanced contractility but not hypertrophy, despite evidence implicating this Gαq/11-coupled receptor in hypertrophy. Contractility, calcium
Null mutation in macrophage migration inhibitory factor prevents muscle cell loss and fibrosis in partial bladder outlet obstruction.
John A. Taylor
American Journal of Physiology: Renal Physiology, 291(6), F1343-F1353 (2006)
T Jindo et al.
Toxicologic pathology, 29(6), 607-616 (2002-01-17)
Methoxyacetic acid (MAA) is a major metabolite of ethylene glycol monomethyl ether (EGME). Previous investigations of the testicular lesion induced by EGME have found that dividing meiotic cells are the most sensitive, although several stages of spermatocytes are also vulnerable.
Daniel A Emmert et al.
American journal of physiology. Cell physiology, 286(1), C8-21 (2003-09-12)
Thus far, determining the relative contribution of Ca2+/calmodulin-dependent myosin light chain kinase (MLCK) and Ca2+-independent Rho-kinase pathways to myosin II activation and contraction has been difficult. In this study, we characterize the role of Rho-kinase in a rat embryo fibroblast
Elena Kassianidou et al.
Molecular biology of the cell, 28(26), 3832-3843 (2017-10-20)
The assembly and mechanics of actomyosin stress fibers (SFs) depend on myosin regulatory light chain (RLC) phosphorylation, which is driven by myosin light chain kinase (MLCK) and Rho-associated kinase (ROCK). Although previous work suggests that MLCK and ROCK control distinct
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