Ugrás a tartalomra
Merck

G3042

Sigma-Aldrich

Anti-GAL4 DNA-BD antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Szinonimák:

Anti-Galactose 4 DNA Binding Domain

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352203
NACRES:
NA.56

biológiai forrás

rabbit

Minőségi szint

konjugátum

unconjugated

antitest forma

affinity isolated antibody

antitest terméktípus

primary antibodies

klón

polyclonal

form

buffered aqueous solution

technika/technikák

western blot: 1-2 μg/mL using GAL4 (DBD 1-147) fusion protein

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Általános leírás

The polyclonal Anti-GAL4 DNA-BD rabbit antibody is a synthetic peptide that recognizes GAL4 DNA binding domain fusion proteins. GAL4 protein is an 881 amino acid transcription factor. It contains functionally independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a constituent of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).

Egyediség

Anti-GAL4 DNA-BD recognizes GAL4 DNA binding domain fusion proteins.

Immunogen

synthetic peptide corresponding to the Saccharomyces cerevisiae GAL4 protein DNA binding domain (amino acids 39-52).

Alkalmazás

Anti-GAL4 DNA-BD antibody produced in rabbit has been used in western blotting.

Biokémiai/fiziológiai hatások

GAL4 transcription factor is involved in the induction of genes that regulate galactose metabolism in Saccharomyces cerevisiae. The yeast two hybrid system is widely used for the characterization of domains that are necessary and sufficient for the interaction of two known proteins by deletion and mutational analysis.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Tárolás és stabilitás

For continuous use store at 2-8°C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Jogi nyilatkozat

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Nem találja a megfelelő terméket?  

Próbálja ki a Termékválasztó eszköz. eszközt

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

nwg

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Anil K Panigrahi et al.
Proceedings of the National Academy of Sciences of the United States of America, 120(4), e2216436120-e2216436120 (2023-01-20)
Enhancers not only activate target promoters to stimulate messenger RNA (mRNA) synthesis, but they themselves also undergo transcription to produce enhancer RNAs (eRNAs), the significance of which is not well understood. Transcription at the participating enhancer-promoter pair appears coordinated, but
Sirtuin 7-dependent deacetylation of DDB1 regulates the expression of nuclear receptor TR4
Karim Mdf, et al.
Biochemical and biophysical research communications, 490(2), 423-428 (2017)
SIRT7 regulates the nuclear export of NF-$\kappa$B p65 by deacetylating Ran
Sobuz SU, et al.
Biochimica et biophysica acta. General subjects, 1866(9), 1355-1367 (2019)
Alexandre Nore et al.
Nature communications, 13(1), 7048-7048 (2022-11-18)
Meiosis requires the formation of programmed DNA double strand breaks (DSBs), essential for fertility and for generating genetic diversity. DSBs are induced by the catalytic activity of the TOPOVIL complex formed by SPO11 and TOPOVIBL. To ensure genomic integrity, DNA
Mitsuki Uemura et al.
Veterinary and comparative oncology, 18(2), 247-255 (2019-09-14)
RAD51 forms a complex with BRCA2 and plays a central role in the DNA damage response pathway that is associated with homologous recombination. The structures of RAD51 and its homologues are highly conserved from prokaryotes to higher eukaryotes. Although a

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással