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D8187

Sigma-Aldrich

JumpStart™ REDTaq® DNA Polymerase

Hot-start Taq enzyme with inert dye, 10X buffer included

Szinonimák:

Hot start DNA polymerase, Hot start Taq

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)

About This Item

MDL-szám:
MFCD00166026
UNSPSC kód:
12352204
NACRES:
NA.55

Minőségi szint

200

Forma

liquid

használat

sufficient for 250 reactions
 sufficient for 2500 reactions
 sufficient for 50 reactions

Jellemzők

dNTPs included: no
hotstart

koncentráció

1 unit/μL

technika/technikák

PCR: suitable

szín

red

bemenet

purified DNA

alkalmasság

suitable for PCR

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

Általános leírás

JumpStart™ REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.

Alkalmazás

JumpStart™ REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) for the amplification of:
  • insulin-enterotoxin ricin fusion gene (INS-RTB)
  • endothelial cells DNA derived from reverse transcribed RNA
  • leg genomic DNA from cricket flies
  • mitochondrial gene by conventional PCR
JumpStart™ REDTaq® DNA Polymerase is also suitable for DNA methylation analysis.

Tulajdonságok és előnyök

  • Reduces non-specific amplification
  • Increased target yield and specificity
  • Higher the amplification irrespective of the target concentration
  • Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
  • Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
  • Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
  • Assembled PCR reactions can be placed at room temperature for up to 2 hours

Kiszerelés

The enzyme is provided with an optimized 10× reaction buffer.

Egység definíció

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Egyéb megjegyzések

View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.

Jogi információk

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

kapcsolódó termék

Product No.
Leírás
Árazás

D7170

2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, 10 mM in H2O

T7791

Thymidine 5′-triphosphate sodium salt solution, 10 mM (pH 7.0)

D7045

2′-Deoxycytidine 5′-triphosphate disodium salt, 10 mM, aqueous solution

D8418

Dimethyl sulfoxide, for molecular biology

M8662

Mineral oil, PCR Reagent

D6920

2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 10 mM

B0300

Betaine solution, 5 M, PCR Reagent

W1754

Water, PCR Reagent

D4184

JumpStart™ Taq DNA Polymerase, without MgCl2

P2893

JumpStart™ Taq ReadyMix™, Complete optimized reagent for hot-start PCR at 2X concentration

D7295

Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

D9307

JumpStart™ Taq DNA Polymerase, with MgCl2

P1107

JumpStart™ REDTaq® ReadyMix™ Reaction Mix, for High-throughput PCR of complex templates

Piktogramok

Exclamation mark
GHS07

Figyelmeztetés

Warning

Figyelmeztető mondatok

H315,H319,H335

Veszélyességi osztályok

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Célzott szervek

Respiratory system

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000331222
SLCR0623
SLCR0624
SLCQ3353
SLCQ2194

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COA keresése

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Expression of a ricin toxin B subunit: insulin fusion protein in edible plant tissues
Carter JE, et al.
Molecular Biotechnology, 44(2), 90-100 (2010)
Contributions of VEGF to age-dependent transmural gradients in contractile protein expression in ovine carotid arteries
Butler SM, et al.
American Journal of Physiology. Cell Physiology, 301(3), C653-C666 (2011)
Sarah D Burnett et al.
Journal of toxicology and environmental health. Part A, 84(24), 1020-1039 (2021-08-25)
Inter-species differences in toxicodynamics are often a critical source of uncertainty in safety evaluations and typically dealt with using default adjustment factors. In vitro studies that use cells from different species demonstrated some success for estimating the relationships between life
Billions and billions sold: pet-feeder crickets (Orthoptera: Gryllidae), commercial cricket farms, an epizootic densovirus, and government regulations make for a potential disaster
Weissman DB, et al.
Zootaxa, 3504(1), 67-88 (2012)
The field cricket Gryllus assimilis and two new sister species (Orthoptera: Gryllidae)
Weissman DB, et al.
Annals of the Entomological Society of America, 102(3), 367-380 (2009)
View All Related Papers

Cikkek

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Protocols

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

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