HCT 116 Cell Line human

91091005, human colon, Epithelial-like

• Szinonimák: HCT116 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• Terméknév: HCT 116 Cell Line human, 91091005, colon carcinoma
• biológiai forrás: human colon
• növekedési mód: Adherent
• kariotípus: Modal no. 45
• morfológia: Epithelial-like
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human colon carcinoma

Sejtvonal leírása

One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose. Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.

Alkalmazás

HCT 116 Cell Line has been used to:

• study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
• study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labelled siRNA
• determine the effect of PTS (polyamine transport system) in the selective uptake of Quilamine HQ1-44, an iron chelator

Tumourigenicity studies

DNS profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22

Táptalaj

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

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