A549 Cell Line human

86012804, human lung (carcinoma), Epithelial

• Szinonimák: A-549 Cells, A549 Cells, NCI-A549 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: A549 Cell Line human, 86012804, from human lung(carcinoma)
• biológiai forrás: human lung (carcinoma)
• növekedési mód: Adherent
• kariotípus: Hypotriploid
• morfológia: Epithelial
• termékek: Lecithin, High amounts of de-saturated fatty acids.
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human Caucasian lung carcinoma

Sejtvonal leírása

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Alkalmazás

Enzymatic studies

Human A549 cell line has been used:

• to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
• to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
• to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

DNS profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Táptalaj

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or tTrypsin/EDTA; 5% CO₂; 37°C.

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