MCF7 Cell Line human

86012803, human breast (adenocarcinoma), Epithelial-like

• Szinonimák: IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• UNSPSC kód: 41106514
• eCl@ss: 32190102

Tulajdonságok

• Terméknév: MCF7 Cell Line human, from human breast(adenocarcinoma), 86012803
• biológiai forrás: human breast (adenocarcinoma)
• növekedési mód: Adherent
• kariotípus: 2n = 46, hypertriploid to hypotetraploid
• morfológia: Epithelial-like
• termékek: Not specified
• receptorok: Reported to express the oestrogen and progesterone receptor
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human Caucasian breast adenocarcinoma

Sejtvonal leírása

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Alkalmazás

Tumourigenicity and virus studies: Type B and C viruses

DNS profil

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Táptalaj

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

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