Összes fotó(3)
Fontos dokumentumok
B2518
Albumin, Acetylated from bovine serum
lyophilized powder, suitable for microbiology
Szinonimák:
Ac-BSA, Acetyl BSA, Acetylated BSA
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
Javasolt termékek
biológiai forrás
bovine
Minőségi szint
Forma
lyophilized powder
tisztítva
heat shock fractionation
eredet
USA origin
technika/technikák
Southern blotting: suitable
blocking: suitable
cell culture | mammalian: suitable
szennyeződések
HIV I and HIVII, HCV and HBsAg, tested negative
pH
7
alkalmasság
suitable for microbiology
UniProt elérési szám
idegen aktivitás
DNase free
DNase, RNase, NICKase, protease, none detected
NICKase free
Protease free
RNase free
tárolási hőmérséklet
2-8°C
Géninformáció
bovine ... ALB(280717)
Looking for similar products? Látogasson el ide Útmutató a termékösszehasonlításhoz
Általános leírás
Native bovine serum albumin (BSA) is a 66 kDa protein, which belongs to the family of serum albumin. It consists of three domains (I, II and III) and is the major protein found in circulatory system. Native BSA is an α-helical, globular and non-glycosylated protein, with 17-disulfide bonds.
Acetylation of bovine serum albumin modifies various residues, most notably lysine (Lys), and also serine (Ser) and threonine (Thr) to lesser extents. MALDI-TOF mass spectrometry studies on acetylated BSA have indicated a MW value about 3000 Da higher compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. Acetylation of BSA also modifies parallel amino acid residues in any trace contaminating nucleases in the BSA, which inactivates that trace nuclease content. Thus acetylated BSA can be used in molecular biology applications where trace nuclease activity must be minimized.
Acetylation of bovine serum albumin modifies various residues, most notably lysine (Lys), and also serine (Ser) and threonine (Thr) to lesser extents. MALDI-TOF mass spectrometry studies on acetylated BSA have indicated a MW value about 3000 Da higher compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. Acetylation of BSA also modifies parallel amino acid residues in any trace contaminating nucleases in the BSA, which inactivates that trace nuclease content. Thus acetylated BSA can be used in molecular biology applications where trace nuclease activity must be minimized.
Alkalmazás
Acetylated bovine serum albumin has been used:
- as a component of protease buffer for protease inhibition assay
- as a blocking agent in the preparation of cDNA glass array
- to select hybridoma antibodies sensitive to M-ficolin epitopes by immunofluorometric assay
This B2518 acetylated BSA product has been cited in various publications and protocols, such as the following:
* As an electrophoresis standard: Lindberg, T. et al., J. Pediatr. Gastroenterol. Nutr., 27(1), 30-36 (1998)
* In in situ end-labeling buffer for labeling of DNA fragments: Save, V. et al., ”Detecting and Quantifying Apoptosis in Tissue Sections”, in Apoptosis Methods and Protocols (H.J.M. Brady, ed.). Methods Mol. Biol., Vol. 282, pp. 67-84 (2004)
* In EMSA studies on nucleocytoplasmic shuttling: Ghosh, C.C. et al., “Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes”, in Membrane Trafficking (A. Vancura, ed.). Methods Mol. Biol., Vol. 457, pp. 279-292 (2008)
* In selection buffer for ribosome display studies: Larman, H.B. et al., Nat. Protoc., 9(1), 90-103 (2014)
* As an electrophoresis standard: Lindberg, T. et al., J. Pediatr. Gastroenterol. Nutr., 27(1), 30-36 (1998)
* In in situ end-labeling buffer for labeling of DNA fragments: Save, V. et al., ”Detecting and Quantifying Apoptosis in Tissue Sections”, in Apoptosis Methods and Protocols (H.J.M. Brady, ed.). Methods Mol. Biol., Vol. 282, pp. 67-84 (2004)
* In EMSA studies on nucleocytoplasmic shuttling: Ghosh, C.C. et al., “Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes”, in Membrane Trafficking (A. Vancura, ed.). Methods Mol. Biol., Vol. 457, pp. 279-292 (2008)
* In selection buffer for ribosome display studies: Larman, H.B. et al., Nat. Protoc., 9(1), 90-103 (2014)
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.
Biokémiai/fiziológiai hatások
Native bovine serum albumin (BSA) acts as a transporter of drugs, hormones and fatty acids. It has calcium binding sites and regulates metal homeostasis.
Tulajdonságok és előnyök
- Acetylated to inactivate trace nucleases
- DNase free / Exonuclease free
- Nickase free / Endonuclease free
- RNase free
- Protease free
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
Válasszon a legfrissebb verziók közül:
Analitikai tanúsítványok (COA)
Lot/Batch Number
Nem találja a megfelelő verziót?
Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Cluster cytometry for high-capacity bioanalysis
Edwards BS, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 81(5), 419-429 (2012)
Characteristics and biological variations of M-ficolin, a pattern recognition molecule, in plasma
Wittenborn T, et al.
Journal of Innate Immunity, 2(2), 167-180 (2010)
Jakob Appel Østergaard et al.
Scientific reports, 11(1), 8919-8919 (2021-04-28)
H-ficolin recognizes patterns on microorganisms and stressed cells and can activate the lectin pathway of the complement system. We aimed to assess H-ficolin in relation to the progression of diabetic kidney disease (DKD), all-cause mortality, diabetes-related mortality, and cardiovascular events.
Julie Brogaard Larsen et al.
Thrombosis and haemostasis, 119(6), 952-961 (2019-04-16)
Activation of the complement system is part of the dysregulated immune response in sepsis. The mannose-binding lectin-associated serine proteases (MASP)-1 and -2 activate the lectin pathway of the complement system. Besides, these proteins can activate coagulation in vitro. However, the
S A Fiscus et al.
Methods in molecular medicine, 20, 209-216 (1999-01-01)
Semen is the major vehicle for the sexual transmission of HIV-1. The ability to isolate infectious HIV from the semen and to quantitate viral burden in the form of cell-free or cell-associated HIV-1 RNA in semen are important for epidemiologic
Protocols
Preparation for biodegradable nanoparticles and their use in transfection protocols .
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással