Ugrás a tartalomra
Merck
Összes fotó(2)

Fontos dokumentumok

A9521

Sigma-Aldrich

Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody produced in rabbit

IgG fraction of antiserum, lyophilized powder

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352203
NACRES:
NA.46
konjugátum:
unconjugated
application:
ELISA (i)
IEP
klón:
polyclonal
faj reaktivitás:
yeast
citations:
171
technika/technikák:
immunoelectrophoresis: suitable
indirect ELISA: 1:15,000-1:30,000

biológiai forrás

rabbit

Minőségi szint

konjugátum

unconjugated

antitest forma

IgG fraction of antiserum

antitest terméktípus

primary antibodies

klón

polyclonal

Forma

lyophilized powder

faj reaktivitás

yeast

technika/technikák

immunoelectrophoresis: suitable
indirect ELISA: 1:15,000-1:30,000

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

célzott transzláció utáni módosítás

unmodified

Általános leírás

G-6-PDH catalyzes the NADP+(or NAD+)-dependent oxidation of D-glucose-6-phosphate to 6-phospho-D-glucono-1,5-lactone. This reaction is the rate limiting step of the pentose phosphate pathway.
Yeast G-6-PDH is often used as a loading control in several immunoassays.
Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme. It synthesizes NADPH (nicotinamide adenine dinucleotide phosphate). The G6PD gene is located on human chromosome X. It spans 18.5 kb and has 13 exons.
The product is specific for G-6-PDH as determined by immunoelectrophoresis. No reaction is observed with other proteins in a crude Baker′s yeast extract.

Immunogén

G-6-PDH from Baker′s yeast S. cerevisiae.

Alkalmazás

Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody has been used to detect the immunoreactive mass of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). It has also been used in western blotting.
Rabbit Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody has been used for western blot assays. The antibody can also be used for indirect ELISA (1:15,000-1:30,000) and immunoelectrophoresis.
Yeast cell extracts were analyzed by western blot and equal loading was determined by probing with rabbit anti-Glucose-6-Phosphate Dehydrogenase antibody.

Biokémiai/fiziológiai hatások

NADPH (nicotinamide adenine dinucleotide phosphate), produced by glucose-6-phosphate dehydrogenase (G6PD) is an electron donor molecule, that helps in neutralizing harmful oxidizing agents. Deficiency in G6PD shows symptoms, that includes neonatal jaundice and acute episodic hemolysis. This enzyme guards the red blood cells against oxidative challenges.

Fizikai forma

Lyophilized from 0.01 M phosphate buffered saline.

Feloldás

Reconstitute with 2mL deionized water.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Nem találja a megfelelő terméket?  

Próbálja ki a Termékválasztó eszköz. eszközt

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number

Nem találja a megfelelő verziót?

Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Ming Li et al.
The Journal of cell biology, 211(3), 639-652 (2015-11-04)
Cells must regulate the abundance and activity of numerous nutrient transporters in different organelle membranes to achieve nutrient homeostasis. As the recycling center and major storage organelle, lysosomes are essential for maintaining nutrient homeostasis. However, very little is known about
Danazol inhibits aromatase activity of endometriosis-derived stromal cells by a competitive mechanism
Murakami K, et al.
Fertility and Sterility, 86(2), 291-297 (2006)
Decreased expression of a member of the Rho GTPase family, Cdc42Hs, in cells from Tangier disease-the small G protein may play a role in cholesterol efflux
Hirano K I, et al.
Febs Letters, 484(3), 275-279 (2000)
Nadya Morozova et al.
Nature cell biology, 8(11), 1263-1269 (2006-10-17)
Ypt-Rab GTPases are key regulators of the various steps of intracellular trafficking. Guanine nucleotide-exchange factors (GEFs) regulate the conversion of Ypt-Rabs to the GTP-bound state, in which they interact with effectors that mediate all the known aspects of vesicular transport.
Cytochemical flow analysis of intracellular G6 PD and aggregate analysis of mosaic G6 PD expression
Kalnoky M, et al.
European Journal of Haematology, 100(3), 294-303 (2018)

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással