A8580

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

• Szinonimák: Anti-Glutathione-S-Transferase, Anti-GST
• UNSPSC kód: 12352203
• NACRES: NA.46

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: agarose conjugate
• antitest forma: IgG fraction of antiserum
• antitest terméktípus: primary antibodies
• klón: polyclonal
• form: PBS suspension
• molekulatömeg: antigen 27.5 kDa
• nem léphet reakcióba: porcine, bovine, human, rabbit, rat
• technika/technikák: ELISA: suitable; immunoprecipitation (IP): suitable
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... GSTP1(2950)

Leírás

Általános leírás

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Alkalmazás

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.

Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Biokémiai/fiziológiai hatások

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Fizikai forma

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Elkészítési megjegyzés

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable