Ugrás a tartalomra
Merck
Összes fotó(5)

Fontos dokumentumok

View All Documentation

G4510

Millipore

Glutathione−Agarose

lyophilized powder

Szinonimák:

Glutathione-Agarose Resin, GSH-agarose, S-linked glutathione agarose

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(5)

About This Item

MDL-szám:
MFCD00131200
UNSPSC kód:
41106500
NACRES:
NA.56

form

lyophilized powder

Minőségi szint

200

analit kémiai osztály(ok)

proteins (GST)

a címkézés mértéke

≥10 μmol per mL gel

technika/technikák

immunoprecipitation (IP): suitable
protein purification: suitable

matrix

4% cross-linked beaded agarose

mátrix aktiválás

epoxy

mátrix csatolmány

sulfur

mátrix távtartó

12 atoms (10 carbon)

tárolási hőmérséklet

−20°C

Looking for similar products? Látogasson el ide Útmutató a termékösszehasonlításhoz

Általános leírás

Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose.

Alkalmazás

Glutathione-Agarose has been used:
  • to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P
  • to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288)
  • in affinity purification of the fusion protein

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Biokémiai/fiziológiai hatások

Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma.

Tulajdonságok és előnyök

  • Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes.
  • Has a shelf life of two years if stored properly desiccated at -20 °C.
  • Binding capacity: 5-10 mg glutathione S-transferase per mL resin

Mennyiség

One g powder swells to ≥10 ml of gel

Fizikai forma

Lyophilized powder stabilized with lactose

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Az ügyfelek ezeket is megtekintették

Slide 1 of 4

1 of 4

Glutathione Seph High Performance Cytiva 17-5279-02, pack of 100 mL

GE17-5279-02

Glutathione Seph High Performance

GST•Bind Resin

Millipore

70541

GST•Bind Resin

L-Glutathione reduced ≥98.0%

Sigma-Aldrich

G4251

L-Glutathione reduced

GST GraviTrap™ Cytiva 28-9523-60

GE28-9523-60

GST GraviTrap™

Wenrong Tan et al.
PLoS genetics, 14(4), e1007336-e1007336 (2018-04-17)
Drought is a major threat to plant growth and crop productivity. The phytohormone abscisic acid (ABA) plays a critical role in plant response to drought stress. Although ABA signaling-mediated drought tolerance has been widely investigated in Arabidopsis thaliana, the feedback
Dustin L Johnson et al.
Journal of bacteriology, 190(8), 2972-2980 (2008-02-19)
Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intracellular
Magdalena M Grabowska et al.
Molecular endocrinology (Baltimore, Md.), 28(6), 949-964 (2014-05-08)
Androgen receptor (AR) action throughout prostate development and in maintenance of the prostatic epithelium is partly controlled by interactions between AR and forkhead box (FOX) transcription factors, particularly FOXA1. We sought to identity additional FOXA1 binding partners that may mediate
Samie R Jaffrey et al.
Proceedings of the National Academy of Sciences of the United States of America, 99(5), 3199-3204 (2002-02-28)
The specificity of the reactions of nitric oxide (NO) with its neuronal targets is determined in part by the precise localizations of neuronal NO synthase (nNOS) within the cell. The targeting of nNOS is mediated by adapter proteins that interact
Hui Li et al.
Nature communications, 9(1), 2573-2573 (2018-07-04)
The dynamic maintenance of polar domains in the plasma membrane (PM) is critical for many fundamental processes, e.g., polar cell growth and growth guidance but remains poorly characterized. Rapid tip growth of Arabidopsis pollen tubes requires dynamic distribution of active
View All Related Papers

Related Content

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással