71183

Sodium acetate

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

• Szinonimák: Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt
• Lineáris képlet: CH₃COONa
• CAS-szám: 127-09-3
• Molekulatömeg: 82.03
• Beilstein: 3595639
• EC-szám: 204-823-8
• MDL-szám: MFCD00012459
• UNSPSC kód: 12161700
• eCl@ss: 39021906
• PubChem Substance ID: 57652226
• NACRES: NA.25

Tulajdonságok

• grade: for luminescence; for molecular biology
• Minőségi szint: 200
• termékcsalád: BioUltra
• Teszt: ≥99.0% (NT)
• form: solid
• öngyulladási hőmérséklet: 1112 °F
• szennyeződések: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• veszteség: ≤0.5% loss on drying, 150 °C
• pH: 8.5-10.0 (25 °C, 3 M in H₂O)
• pKa: 4.76 (acetic acid)
• mp: >300 °C (dec.) (lit.)
• oldhatóság: H₂O: 3 M at 20 °C, clear, colorless
• anion nyomok: chloride (Cl^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• kation nyomok: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤10 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤10 mg/kg; Zn: ≤5 mg/kg
• SMILES string: [Na+].CC([O-])=O
• λ: 3 M in H₂O
• UV-elnyelés: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.02
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• Nemzetközi kémiai azonosító kulcs: VMHLLURERBWHNL-UHFFFAOYSA-M

Leírás

Alkalmazás

Sodium acetate is suitable for use as a buffer in:

• protein crystallization
• staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
• the preparation of standard (acetylcholine chloride) for hestrin assay
• substrate screening characterization for microfluidic microbial fuel cell array

Biokémiai/fiziológiai hatások

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Egyéb megjegyzések

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)