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Merck

SE100

Sigma-Aldrich

Silver Enhancer Kit

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12171500
NACRES:
NA.47

form

liquid

Minőségi szint

használat

sufficient for ≥100 slides staining and fixing

alkalmazás(ok)

diagnostic assay manufacturing
hematology
histology

tárolási hőmérséklet

2-8°C

Alkalmazás

Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.

Tulajdonságok és előnyök

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Kiszerelés

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Elv

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Kit Components Only

Product No.
Leírás

  • Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g

Kit Components Also Available Separately

Product No.
Leírás
Biztonsági adatlap

  • S5020Silver enhancer solution A 100 mLBiztonsági adatlap

  • S5145Silver enhancer solution B 100 mLBiztonsági adatlap

Piktogramok

Health hazardEnvironment

Figyelmeztetés

Danger

Figyelmeztető mondatok

Óvintézkedésre vonatkozó mondatok

Veszélyességi osztályok

Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B

Tárolási osztály kódja

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


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Dokumentumtár megtekintése

Joanna Seiffert et al.
Respiratory research, 17(1), 85-85 (2016-07-21)
The increasing use of silver nanoparticles (AgNPs) in consumer products is concerning. We examined the potential toxic effects when inhaled in Brown-Norway (BN) rats with a pre-inflammatory state compared to Sprague-Dawley (SD) rats. We determined the effect of AgNPs generated
Catarina Coutinho et al.
Analytical and bioanalytical chemistry, 411(9), 1807-1824 (2018-11-06)
MicroRNAs (miRNAs) are small regulatory RNAs, the dysregulation of which has been associated with the progression of several human diseases, including cancer. Interestingly, these molecules can be used as biomarkers for early disease diagnosis and can be found in a
Yuan Chen et al.
Oncotarget, 9(41), 26556-26571 (2018-06-15)
Tumor hypoxia is a well-recognized driver of resistance to traditional cancer therapies such as chemotherapy and radiation therapy. We describe development of a new nanoconstruct composed of gold nanorods (GNRs) conjugated to carbonic anhydrase IX (CAIX) antibody that specifically binds
F Liu et al.
The Plant cell, 13(5), 1063-1078 (2001-05-08)
Some plant cytoplasms express novel mitochondrial genes that cause male sterility. Nuclear genes that disrupt the accumulation of the corresponding mitochondrial gene products can restore fertility to such plants. The Texas (T) cytoplasm mitochondrial genome of maize expresses a novel
Kathleen Grennan et al.
Talanta, 68(5), 1591-1600 (2008-10-31)
With lower limits of detection and increased stability constantly being demanded of biosensor devices, characterisation of the constituent layers that make up the sensor has become unavoidable, since this is inextricably linked with its performance. This work describe the optimisation

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