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Merck
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Fontos dokumentumok

39465

Millipore

Gelatin from porcine skin

suitable for microbiology, ultrahigh gel strength

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

CAS-szám:
EC-szám:
MDL-szám:
UNSPSC kód:
41106212
NACRES:
NA.85

biológiai forrás

Porcine

Minőségi szint

Forma

powder

minőség

ultrahigh gel strength

eltarthatósági idő

limited shelf life, expiry date on the label

veszteség

9.5-12.5% loss on drying

áteresztőképesség

450 nm, ≥85%
620 nm, ≥95%

pH

5.20-5.60

vezetőképesség

120-190 μS/cm at 30 °C (1%)

viszkozitás

5.10-5.80 mPa.s

gél szilárdság

280-302 g BloomAOAC

oldhatóság

H2O: 67 mg/mL at 60 °C

kation nyomok

Ca: ≤100 mg/kg

alkalmazás(ok)

microbiology

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Alkalmazás

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Komponensek

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Vigyázat

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Elkészítési megjegyzés

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentrat ion. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. Manufactured by Gelita AG

Tárolási osztály kódja

11 - Combustible Solids

WGK

nwg

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


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Analitikai tanúsítványok (COA)

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Dokumentumtár megtekintése

Johno Breeze et al.
Journal of the Royal Army Medical Corps, 159(2), 84-89 (2013-05-31)
Ballistic gelatin is the most common tissue simulant used to reproduce the penetration of projectiles into muscle but published data to support its use are primarily based on bullets, despite explosive fragments being the most common cause of injury to
M V Nasekin et al.
Anesteziologiia i reanimatologiia, (1)(1), 59-60 (2013-07-03)
The article deals with skills improving problem of epidural anaesthesia with ultrasound control. Methods of gelatin spinal column model making, use and its economical side are discussed in the article.
Ivy Kanungo et al.
Materials science & engineering. C, Materials for biological applications, 33(4), 2455-2459 (2013-03-19)
The dielectric behavior of the gelatine-GAGs based blend systems has been studied to understand the dynamic behavior of the water at the protein-GAGs interfaces which are relevant for tissue engineering application. Impedance (Z) and phase have been measured as a
Panuphong Pootawang et al.
Journal of nanoscience and nanotechnology, 13(1), 589-592 (2013-05-08)
The environmental concern pays much attention to the recent cause of the global warming effect. The reduction of the chemical uses is one of many ways to avoid this crucial problem. Herein, the green process for silver nanometallic particle formation
Kimberly E Hawkins et al.
Molecular brain, 6, 14-14 (2013-03-26)
Matrix metalloproteinases are important factors in the molecular mechanisms leading to neuronal injury in many neurological disorders. Matrix metalloproteinase (MMP)-9 is up-regulated after cerebral ischemia and neuroinflammation and is actively involved in blood-brain barrier disruption. Current methods of measuring MMP-9

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