Ugrás a tartalomra
Merck
Összes fotó(3)

Fontos dokumentumok

48722

Millipore

Gelatin from porcine skin

medium gel strength, suitable for microbiology

Szinonimák:

Gelatine, hydrolyzed collagen, collagen hydrolysate, gelatine hydrolysate

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

CAS-szám:
EC-szám:
MDL-szám:
UNSPSC kód:
41106212
NACRES:
NA.85

biológiai forrás

Porcine

Minőségi szint

sterilitás

non-sterile

Forma

powder

minőség

medium gel strength

kiszerelés

bottle of 100 g
bottle of 500 g

izzítási maradék

≤2%

veszteség

≤15% loss on drying

szín

light yellow

pH

4.0-6.0 (25 °C, 67 mg/mL in H2O)

gél szilárdság

165-195 g Bloom (67 mg/ml water)

oldhatóság

H2O: 67 mg/mL at 50 °C, slightly hazy, slightly yellow

anion nyomok

chloride (Cl-): ≤3000 mg/kg

kation nyomok

Ca: ≤2000 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤10 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
K: ≤500 mg/kg
Mg: ≤500 mg/kg
Mn: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤10 mg/kg

alkalmazás(ok)

microbiology

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Általános leírás

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred to as type A. It is a hydrocolloid and is rich in glycine, proline, and hydroxyproline, which impart structural stability. It is synthesized from the alkaline digestion of collagen from porcine. Gelatin takes up a random coil structure after digestion from the triple helical collagen.

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc., in water. Type A gelatin is derived from acid-cured tissue, and Type B from lime-cured tissue.

The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 180.

Alkalmazás

Gelatin has been used in many applications, such as in coating cell culture plates to improve attachment of cells, in PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology.

Komponensek

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Vigyázat

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Elkészítési megjegyzés

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. Manufactured by Gelita AG

Tárolási osztály kódja

11 - Combustible Solids

WGK

nwg

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


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