COLLP-RO

Collagenase P

from Clostridium histolyticum

• Enzyme Commission szám: 3.4.24.3 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: Clostridium histolyticum
• Minőségi szint: 100
• sterilitás: non-sterile
• Forma: lyophilized
• collagenase activity: >1.5 U/mg (Lyophilizate, Collagenase activity)
• kiszerelés: pkg of 1 g (11249002001); pkg of 100 mg (11213857001); pkg of 2.5 g (11213873001); pkg of 500 mg (11213865001)
• gyártó/kereskedő neve: Roche
• koncentráció: 0.5-2.5 mg/mL
• technika/technikák: tissue processing: suitable
• szín: light brown to brown
• oldhatóság: water: soluble
• NCBI elérési szám: WP_138210331
• alkalmazás(ok): life science and biopharma
• idegen aktivitás: Clostripain 24.099 U/mg; Protease 4.4 U/mg (Proteases); Trypsin 53.428 U/mg (with BAEE)
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Collagenase P is an enzyme mixture prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.Collagenase P is a research biochemical, not manufactured under the US good manufacturing practices (GMP) regulations.
Note: Collagenase P is a non-sterile preparation of the culture supernatant of Clostridium histolyticum. It is neither designed nor intended for isolation of pancreatic islets for transplantation into humans and must not be used for such purposes.

Egyediség

Enzyme activity:
Collagenase activity: >1.5U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase P has a very high collagenase activity (>1.5U/mg), and is function tested for the isolation of mouse pancreatic islets.
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.

Alkalmazás

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats.

Tulajdonságok és előnyök

Contents:
Lyophilizate, nonsterile

Elkészítési megjegyzés

Activator: Ca²⁺
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Working concentration: 0.5 to 2.5mg/ml
0.5 to 1.5mg/ml for the isolation of pancreatic islets from mouse and rat pancreas.
Approximately 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C. Prepare appropriate aliquots and avoid repeated freezing and thawing.

Feloldás

Reconstitution in any balanced salt solution (e.g., HBSS)

Tárolás és stabilitás

Store dry

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Piktogramok: GHS07,GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315 - H319 - H334 - H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Veszélyességi osztályok: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash