COLLH-RO

Collagenase H

from Clostridium histolyticum

• Enzyme Commission szám: 3.4.24.3 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: Clostridium histolyticum
• Minőségi szint: 100
• sterilitás: non-sterile
• form: lyophilized
• collagenase activity: 0.15 U/mg lyophilizate
• kiszerelés: pkg of 100 mg (11074032001); pkg of 2.5 g (11087789001); pkg of 500 mg (11074059001)
• gyártó/kereskedő neve: Roche
• koncentráció: ~1 mg/mL (Isolation of rat hepatocytes); ~2 mg/mL (Isolation of adipocytes)
• technika/technikák: tissue processing: suitable
• szín: brown
• optimális pH: 6.0-8.0
• oldhatóság: water: soluble
• NCBI elérési szám: WP_138210331
• UniProt elérési szám: Q46085
• alkalmazás(ok): life science and biopharma
• idegen aktivitás: Clostripain 39.798 U/mg; Proteases 14.014 U/mg (Azocoll, based on lyoph); Trypsin 1.668 U/mg (with BAEE)
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Collagenase H is an enzyme mixture prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.

Egyediség

Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.

Alkalmazás

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.

Fizikai forma

Lyophilizate, nonsterile

Elkészítési megjegyzés

Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator: Ca²⁺
Working concentration: 0.5 to 2.5mg/ml,
Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.

Feloldás

Reconstitution in any balanced salt solution (e.g., HBSS)

Tárolás és stabilitás

Keep container tightly closed in a dry and well-ventilated place.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Piktogramok: GHS07,GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315 - H319 - H334 - H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Veszélyességi osztályok: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash