COLLDISP-RO

Collagenase/Dispase^®

lyophilized, non-sterile, optimum pH 7.0-8.0

• Szinonimák: collagenase
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: enzyme from Bacillus polymyxa ( Dispase ); enzyme from bacterial ( Collagenase from Vibrio alginolyticus)
• Minőségi szint: 100
• sterilitás: non-sterile
• form: lyophilized
• collagenase activity: >0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as substrate)
• specifikus aktivitás: >0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)
• kiszerelés: pkg of 100 mg (10269638001); pkg of 500 mg (11097113001)
• gyártó/kereskedő neve: Roche
• koncentráció: 1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
• technika/technikák: tissue processing: suitable
• szín: light yellow to brown
• optimális pH: 7.0-8.0
• oldhatóság: water: soluble
• UniProt elérési szám: P43154
• alkalmazás(ok): life science and biopharma; sample preparation
• idegen aktivitás: proteolytic activities <0.1 U/mg (hemoglobin as the substrate)
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact. Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C.

Egyediség

Collagenase degrades native collagen. Dispase^® is a nonspecific protease.

Alkalmazás

Collagenase/Dispase^® is used for the preparation of cells from a wide variety of different tissues and organs.

Komponensek

EC 3.4.24.4 & 3.4.24.3

Egység definíció

One unit is the enzyme activity, which liberates under assay conditions within 1 minute Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine.

Elkészítési megjegyzés

Activator: Ca²⁺

Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)

Enzyme activity:
Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Dispase^®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5)
Contaminating proteolytic activities: <0.1U/mg lyophilizate (hemoglobin as the substrate)

Working solution: Preparation of working solution Dissolve enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca²⁺/Mg²⁺-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Enzyme concentration of the sterile solution: Collagenase, 0.1U/mL PBS; Dispase^®, 0.8U/mL PBS.

Inhibitors:
Collagenase inhibitors: EDTA, EGTA
Collagenase/Dispase is not inhibited by serum.

Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C.
Note: Store in appropriate aliquots and avoid repeated freezing and thawing!

Feloldás

Reconstitution in water (100mg/mL). Further dilution with PBS (phosphate buffered saline) (Ca²⁺/Mg²⁺-free).

Tárolás és stabilitás

Store dry

Egyéb megjegyzések

Collagenase from Vibrio alginolyticus, Dispase^® from Bacillus polymyxa

For life science research only. Not for use in diagnostic procedures.

Jogi információk

Dispase is a registered trademark of Godo Shusei Co., Ltd.

Biztonsági adatok

• Piktogramok: GHS07,GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315 - H319 - H334 - H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Veszélyességi osztályok: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash