C8051

Collagenase from Clostridium histolyticum

Sigma Blend Type H, ≥1.0 FALGPA units/mg solid

• Szinonimák: Clostridiopeptidase A
• CAS-szám: 9001-12-1
• Enzyme Commission szám: 3.4.24.3 (BRENDA, IUBMB)
• EC-szám: 232-582-9
• MDL-szám: MFCD00130830
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• Minőségi szint: 200
• form: powder
• caseinase activity: ≥10 units/mg solid
• specifikus aktivitás: ≥1.0 FALGPA units/mg solid
• molekulatömeg: 68-130 kDa
• tárolási hőmérséklet: −20°C

Leírás

Alkalmazás

Collagenase from Clostridium histolyticum has been used:

• to harvest osteosarcoma 143B cells from collagen scaffold
• in tissue digestion of islets and carotid artery
• in the digestion of chorionic villi
• in enzymatic dissociation for ATP-based tumour chemosensitivity assay (ATP-TCA) in solid tumors

Biokémiai/fiziológiai hatások

Clostridium histolyticum produces two classes of collagenases. Clostridial collagenases are found to be highly effective than mammalian collagenases. It is capable of cleaving at multiple cleavage sites within the collagen triple helix. It is implicated in the bacterial invasion in gas gangrene.

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL is used for digestion. The duration of reaction varyies from 15 minutes to several hours to yield a satisfactory efficiency of cell dissociation, without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preffered and Zn²⁺ is required for activity. This enzyme is tested for the release of hepatocytes at approximately 1 mg/mL conc., in a total volume of 100 mL for each rat liver.

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Egység definíció

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Biztonsági adatok

• Piktogramok: GHS08,GHS07
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315,H319,H334,H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Faceshields, Gloves