11585029001

NBT

crystals, pkg of 5 g

• Szinonimák: Nitrotetrazolium Blue chloride, 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT
• Lineáris képlet: C₄₀H₃₀N₁₀O₆ · 2Cl
• CAS-szám: 298-83-9
• Molekulatömeg: 817.64
• Beilstein: 4115923
• MDL-szám: MFCD00012159
• UNSPSC kód: 41116100
• PubChem Substance ID: 329749491

Tulajdonságok

• leírás: 4-Nitro blue tetrazolium chloride, crystals
• Minőségi szint: 100
• Forma: crystals
• kiszerelés: pkg of 5 g
• gyártó/kereskedő neve: Roche
• tárolási hőmérséklet: 2-8°C
• SMILES string: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• Nemzetközi kémiai azonosító kulcs: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Leírás

Általános leírás

NBT is soluble in water, aqueous buffers, and dimethylformamide.

Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI. Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications. It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Alkalmazás

• Colony and plaque hybridization
• Immunohistocytochemistry
• In situ hybridization
• Northern blot
• Southern blot
• Western blot

NBT has been used in in situ hybridization.

Minőség

Performance-controlled in the LDH assay.

Elkészítési megjegyzés

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.

Analízis megjegyzés

Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Piktogramok: GHS07
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H302,H315,H319,H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
• Veszélyességi osztályok: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 2
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash