Ugrás a tartalomra
Merck
Összes fotó(2)

Key Documents

View All Documentation

B1911

Sigma-Aldrich

BCIP®/NBT

alkaline phosphatase substrate, chromogenic, liquid

Szinonimák:

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

UNSPSC kód:
12352204
NACRES:
NA.83

product name

BCIP®/NBT Liquid Substrate System, ready to use solution

Minőségi szint

300

form

liquid

tárolási hőmérséklet

2-8°C

Alkalmazás

A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
  • for staining alkaline phosphatase(ALP) in bone marrow stromal cells
  • as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
  • as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays

Vigyázat

It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.

Jogi információk

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase
Ling M, et al.
Cell & Bioscience, 7(1), 27-27 (2017)
Salma E Jiménez-Badillo et al.
Scientific reports, 7(1), 13351-13351 (2017-10-19)
The Catsper1 gene, whose expression is restricted to male germ cells, has great importance in reproductive biology because of its function in sperm motility and fertilization. We previously reported that the promoter of this gene has transcriptional activity in either
Jungho Jang et al.
Scientific reports, 8(1), 7211-7211 (2018-05-10)
The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified
The evaluation of angiogenesis and matrix metalloproteinase-2 secretion in bone marrow of multiple myeloma patients before and after the treatment
Bolkun L, et al.
Advances in Medical Sciences, 58(1), 118-125 (2013)
Andrzej Swistowski et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(50), 15703-15712 (2009-12-18)
Multiple recent reports implicate amyloid precursor protein (APP) signaling in the pathogenesis of Alzheimer's disease, but the APP-dependent signaling network involved has not been defined. Here, we report a novel consensus sequence for interaction with the PDZ-1 and PDZ-2 domains
View All Related Papers

Cikkek

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Protocols

Immunodetektálás BCIP/NBT szubsztrát használatával

A BCIP/NBT szubsztrát stabil, intenzív színt ad az alkalikus foszfatáz vizsgálatokhoz, ideális vizuális megfigyeléshez.

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással