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Merck
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Fontos dokumentumok

PC205L

Sigma-Aldrich

Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb

lyophilized, Calbiochem®

Szinonimák:

Anti-CGRP

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
NACRES:
NA.43

biológiai forrás

rabbit

Minőségi szint

antitest forma

serum

antitest terméktípus

primary antibodies

klón

polyclonal

form

lyophilized

tartalmaz

≤0.1% sodium azide as preservative

faj reaktivitás

rat, mouse

gyártó/kereskedő neve

Calbiochem®

tárolási körülmény

OK to freeze

izotípus

IgG

kiszállítva

ambient

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

mouse ... Calca(12310)

Általános leírás

Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
Recognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.

Immunogen

rat α-calcitonin gene related peptide (α-CGRP)

Alkalmazás


Free Floating Sections (see application references)
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200, see application references)
Paraffin Sections (see application references; heat pre-treatment required; see comments)

Figyelmeztetés

Toxicity: Standard Handling (A)

Feloldás

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.

Analízis megjegyzés

Positive Control
Rat amygdala or spinal cord

Egyéb megjegyzések

Antibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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