MABF2158
Anti-TMPRSS2 Antibody, clone P5H9-A3
clone P5H9-A3, from mouse
Szinonimák:
Transmembrane protease serine 2, Serine protease 10
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Javasolt termékek
biológiai forrás
mouse
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
P5H9-A3, monoclonal
faj reaktivitás
human
kiszerelés
antibody small pack of 25 μg
technika/technikák
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
izotípus
IgG1κ
UniProt elérési szám
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... TMPRSS2(7113)
Related Categories
Általános leírás
Transmembrane protease serine 2 (UniProt: O15393; also known as Serine protease 10, TMPRSS2) is encoded by the TMPRSS2 (also known as PRSS10) gene (Gene ID: 7113) in human. TMPRSS2 is a single-pass type II membrane protein of the peptidase S1 family that is shown to proteolytically cleave and activate the viral spike glycoproteins, which facilitate virus-cell membrane fusions. It is shown to facilitate human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2 that promotes viral uptake and cleavage of coronavirus spike glycoprotein, which activates the glycoprotein for cathepsin L-independent host cell entry. TMPRSS2 is highly expressed in the prostate tissue and lower expression levels are observed in the epithelia of the colon, stomach, epididymis and breast tissue. Some expression has also been reported in pancreatic acini, hepatic bile ducts, testicular Leydig cells and the kidney. Its expression levels are significantly elevated in both neoplastic prostate and in the epithelium of prostatic hyperplasia. TMPRSS2 has a cytoplasmic domain (aa 1-84), a transmembrane domain (aa 85-105), and an extracellular domain (aa 106-492). Its peptidase S1 domain is localized to amino acids 256-489. It is reported to be proteolytically processed by an autocatalytic mechanism generating the transmembrane protease serine 2 non-catalytic chain (1-255) and the transmembrane protease serine 2 catalytic chain (256-492). Two isoforms of TMPRSS2 have been described that are produced by alternative splicing.
Egyediség
Clone P5H9-A3 specifically detects human Transmembrane protease serine 2. It targets an epitope with in the extracellular serine protease domain.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human transmembrane protease serine 2.
Alkalmazás
Anti-TMPRSS2, clone P5H9-A3, Cat. No. MABF2158, is a mouse monoclonal antibody that detects Transmembrane protease serine 2 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: 1:50 dilution from a representative lot detected TMPRSS2 in human kidney tissue sections.
Enzyme Immunoassay (ELISA) Analysis: A representative lot detected TMPRSS2 in ELISA applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).
Western Blotting Analysis: A representative lot detected TMPRSS2 in Western Blotting applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected TMPRSS2 in Immunohistochemistry applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25; Bertram, S., et. al. (2012). PLoS One. 7(4):e35876).
Enzyme Immunoassay (ELISA) Analysis: A representative lot detected TMPRSS2 in ELISA applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).
Western Blotting Analysis: A representative lot detected TMPRSS2 in Western Blotting applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected TMPRSS2 in Immunohistochemistry applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25; Bertram, S., et. al. (2012). PLoS One. 7(4):e35876).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Minőség
Evaluated by Immunohistochemistry (Paraffin) in human prostate tissue sections.
Immunohistochemistry (Paraffin) Analysis: 1:250 dilution of this antibody detected TMPRSS2 in human prostate tissue sections.
Immunohistochemistry (Paraffin) Analysis: 1:250 dilution of this antibody detected TMPRSS2 in human prostate tissue sections.
Cél megnevezése
53.86 kDa calculated.
Fizikai forma
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Egyéb megjegyzések
Concentration: Please refer to lot specific datasheet.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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