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Merck
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Fontos dokumentumok

MABE419

Sigma-Aldrich

Anti-m3G-cap, m7G-cap Antibody, clone H-20

clone H-20, from mouse

Szinonimák:

Anti-m3G/m7G-cap, Clone H-20 Anti-m3G-cap, m7G-cap Detection Antibody

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
H-20, monoclonal
application:
DB
ICC
IP
WB
technika/technikák:
dot blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
6

biológiai forrás

mouse

Minőségi szint

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

H-20, monoclonal

faj reaktivitás (homológia által előrejelzett)

all

technika/technikák

dot blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotípus

IgG1κ

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Általános leírás

The nucleotide structure named the 2,2,7-trimethylguanosine(m3G)-containing cap structure of small nuclear ribonucleoprotein particles, or U snRNPs is an essential part of mRNA processing. Each snRNP particle consists of one (U1, U2 and U5) or two (U4/U6) snRNA molecules, a common set of eight core proteins (B, B9, D1, D2, D3, E, F and G, also denoted Sm proteins) that are bound to each of the 2,2,7- trimethylguanosine (m3G) cap-containing snRNAs U1, U2, U4 and U5, and several proteins associated specifically with the individual U snRNPs. Except for U6 snRNP, which does not leave the nucleus, the synthesis of these U snRNPs requires the bidirectional transport of the snRNA across the nuclear envelope. The snRNAs U1, U2, U4 and U5 are synthesized in the nucleus with a 7-monomethylguanosine (m7G) cap structure whereas the Sm proteins are stored in the cytoplasm and do not migrate into the nucleus in the absence of bound U snRNA. Instead, newly transcribed U snRNAs are transiently exported into the cytoplasm where the Sm proteins bind the snRNA’s Sm site, to form a ribonucleoprotein complex referred to as the Sm core. Stable association of all Sm proteins is essential for the hypermethylation of the m7G-cap to the m3G-cap structure. After this event and processing of the snRNAs, the mature snRNP particles are transported back to the nucleus in a receptor-and energy-dependent manner and the complete particle is formed. Monoclonal H-20 recognizes both m3G cap containing snRNPs as well as m7G capped structures and it should have a wide application, not only for studying the molecular biology and immunology of the U snRNPs from diverse organisms, but also for the characterization and isolation of m7G-capped transcripts.

Immunogén

Human m3G-cap and m7G-cap

Alkalmazás

Anti-m3G-cap, m7G-cap Antibody, clone H-20 is a mouse monoclonal antibody, validated for use in Dot Blot, IP, western blotting & ICC.
Immunoprecipitation Analysis: A representative lot immunoprecipitated m3G-capped and m7G-capped Xenopus EF-1a, which was spiked into 4 µg of HeLa total RNA.

Western Blotting Analysis: A representative lot from an independent laboratory detected m3G-cap in m3G-capped snRNAs from HeLa nuclear extracts (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated m3G-cap from prefractionated HeLa S100 extracts (Huber, J., et al. (1998). EMBO J. 17(14):4114-4126.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected m3G-cap in m3G-capped U1 snRNPs from digitonin permeabilized HeLa cells (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Minőség

Evaluated by Dot Blot in in vitro transcribed RNA without and with m7G-cap.

Dot Blot Analysis: 1 µg/mL of this antibody detected m7G-cap in in vitro transcribed RNA containing m7G-cap.

Fizikai forma

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Francesco Neri et al.
Nature, 543(7643), 72-77 (2017-02-23)
In mammals, DNA methylation occurs mainly at CpG dinucleotides. Methylation of the promoter suppresses gene expression, but the functional role of gene-body DNA methylation in highly expressed genes has yet to be clarified. Here we show that, in mouse embryonic
Yasutoshi Akiyama et al.
RNA biology, 17(8), 1116-1124 (2020-03-03)
Recent transcriptome-wide studies have identified a diverse pool of transfer RNA (tRNA)-derived RNAs or tRNA-derived fragments (tRFs). Some of these RNAs have been demonstrated to be functional and involved in multiple biological processes ranging from the regulation of gene expression
Sharon Si Jia Ng et al.
iScience, 4, 68-75 (2018-09-22)
Although stable intronic sequence RNAs (sisRNAs) are conserved in plants and animals, their functional significance is still unclear. We identify a pool of polyadenylated maternally deposited sisRNAs in Drosophila melanogaster. These sisRNAs can be generated by independent transcription from the
Abhijit S Deshmukh et al.
Scientific reports, 6, 35288-35288 (2016-10-21)
Cyclin-dependent kinase 7 in conjunction with CyclinH and Mat1 activates cell cycle CDKs and is a part of the general transcription factor TFIIH. Role of Cdk7 is well characterized in model eukaryotes however its relevance in protozoan parasites has not
Alternative cleavage and polyadenylation generates downstream uncapped RNA isoforms with translation potential.
Malka, et al.
Molecular Cell, 82, 3840-3855 (2022)

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