MAB5326

Anti-Nestin Antibody, clone 10C2

clone 10C2, Chemicon^®, from mouse

• Szinonimák: Anti-Nestin
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: 10C2, monoclonal
• application: ICC; IHC (p); WB
• faj reaktivitás: human
• technika/technikák: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• citations: 467

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: purified immunoglobulin
• klón: 10C2, monoclonal
• faj reaktivitás: human
• nem léphet reakcióba: rat, mouse
• kiszerelés: antibody small pack of 25 μg
• gyártó/kereskedő neve: Chemicon^®
• technika/technikák: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• bemenet: sample type epithelial cells; sample type neural stem cell(s)
• izotípus: IgG1
• NCBI elérési szám: NM_006617.1
• UniProt elérési szám: P48681
• kiszállítva: ambient
• tárolási hőmérséklet: 2-8°C
• célzott transzláció utáni módosítás: unmodified

Leírás

Általános leírás

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

Egyediség

Reacts with human Nestin, with weak reactivity to monkey nestin. Not reactive with rodent nestin.

Immunogén

Epitope: human

Fusion protein.

Alkalmazás

Immunoblotting: 1:1,000-1:2,000 on denatured protein. Western blot: 1:1000-1:5000. Recognizes a 220-240kDA band on denaturing SDS gels. There is also a 60-64kDa band present in most lysates, the identity of this smaller fragment is unknown but it is not seen in lysates not containing human nestin protein.

WI-38 (ATCC# CCL75) can be used as a positive control. U251 cell line is also positive.

Immunocytochemistry: 1:200 on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton

Immunohistochemistry: paraformaldehyde is the preferred fixative: 1:200 on formalin fixed, paraffin embedded tissue sections. Use triton 0.02% in block only; dilution buffer 0.1% BSA; incubations 1-2 hours RT. Human fetal brain is suggested as a positive control. (CA Messam et al, Develop. Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002). Filamentous staining in the cytoplasm of cells

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Neural Stem Cells

This Anti-Nestin Antibody, clone 10C2 is validated for use in IC, IH, IH(P), WB for the detection of Nestin.

Cél megnevezése

200-220 kDa

Kapcsolódás

Replaces: AB5922

Fizikai forma

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Protein A purified

Tárolás és stabilitás

Antibodies may be stored at 4°C or -20°C. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Analízis megjegyzés

Control
Positive Control: WI-38 (ATCC# CCL75) or U251 cell lines

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2