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Merck
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MAB4381

Sigma-Aldrich

Anti-TRA-1-81 Antibody, clone TRA-1-81

clone TRA-1-81, Chemicon®, from mouse

Szinonimák:

Clone TRA-1-81 Antibody, TRA-1-81 Detection Antibody

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(5)

About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
TRA-1-81, monoclonal
application:
FACS
ICC
IF
IP
WB
faj reaktivitás:
human
technika/technikák:
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
264

biológiai forrás

mouse

Minőségi szint

100

antitest forma

saturated ammonium sulfate (SAS) precipitated

antitest terméktípus

primary antibodies

klón

TRA-1-81, monoclonal

faj reaktivitás

human

gyártó/kereskedő neve

Chemicon®

technika/technikák

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

bemenet

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

izotípus

IgM

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Related Categories

Általános leírás

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.

Egyediség

This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.

Immunogén

Human embryonal carcinoma cell line 2102Ep

Alkalmazás

Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.
Immunofluorescence: A previous lot of this antibody was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Cél megnevezése

420/250 kDa

Fizikai forma

Format: Purified
Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.

Tárolás és stabilitás

Stable for 1 year at from date of receipt.

Analízis megjegyzés

Control
Human embryonic stem cells

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

nwg

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Expression of pluripotent stem cell markers in the human fetal ovary.
Kerr, CL; Hill, CM; Blumenthal, PD; Gearhart, JD
Human Reproduction null
C Eguizabal et al.
Stem cells (Dayton, Ohio), 29(8), 1186-1195 (2011-06-18)
Gamete failure-derived infertility affects millions of people worldwide; for many patients, gamete donation by unrelated donors is the only available treatment. Embryonic stem cells (ESCs) can differentiate in vitro into germ-like cells, but they are genetically unrelated to the patient.
Tomoko Andoh-Noda et al.
Molecular brain, 8, 31-31 (2015-05-28)
Rett syndrome (RTT) is one of the most prevalent neurodevelopmental disorders in females, caused by de novo mutations in the X-linked methyl CpG-binding protein 2 gene, MECP2. Although abnormal regulation of neuronal genes due to mutant MeCP2 is thought to
Hidehito Saito et al.
Stem cells international, 2016, 8394960-8394960 (2016-04-09)
Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients hold great promise for autologous cell therapies. One of the possible applications of iPSCs is to use them as a cell source for producing autologous lymphocytes for cell-based therapy
Justin K Ichida et al.
Nature chemical biology, 10(8), 632-639 (2014-06-24)
The reprogramming of somatic cells to pluripotency using defined transcription factors holds great promise for biomedicine. However, human reprogramming remains inefficient and relies either on the use of the potentially dangerous oncogenes KLF4 and CMYC or the genetic inhibition of
View All Related Papers

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