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AB5603

Sigma-Aldrich

Anti-SOX2 Antibody

CHEMICON®, rabbit polyclonal

Szinonimák:

Transcription factor SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2, Transcription factor SOX2

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(8)

About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ICC
IF
IHC
WB
faj reaktivitás:
human, mouse
technika/technikák:
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
561

Terméknév

Anti-Sox2 Antibody, Chemicon®, from rabbit

biológiai forrás

rabbit

Minőségi szint

100
300

antitest forma

affinity isolated antibody

antitest terméktípus

primary antibodies

klón

polyclonal

tisztítva

affinity chromatography

faj reaktivitás

human, mouse

faj reaktivitás (homológia által előrejelzett)

rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)

kiszerelés

antibody small pack of 25 μg

gyártó/kereskedő neve

Chemicon®

technika/technikák

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI elérési szám

NP_003097

UniProt elérési szám

P48431

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... SOX2(6657)

Related Categories

Általános leírás

Transcription factor SOX-2 (UniProt: P48431; also known as SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2) is encoded by the SOX2 gene (Gene ID: 6657) in human. SOX-2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is shown to be critical for early embryogenesis and for embryonic stem cell pluripotency. It may function as a switch in neuronal development. It allows neural cells in undifferentiated state by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Sumoylation of SOX-2 is shown to inhibit binding on DNA and negatively regulate the FGF4 transactivation. Defects in SOX2 gene are linked to microphthalmia that is characterized by eye malformation ranging from small eye size of a single eye to complete bilateral absence of ocular tissue.

Egyediség

Anti-Sox2 Antibody recognizes Sox2 C-terminal region.

Immunogén

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2.

Alkalmazás

Anti-SOX2 Antibody, Cat. No. AB5603, is a highly specific rabbit polyclonal antibody SOX2 and has been tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Sox2 in H9 human stem cells.
Immunocytochemistry Analysis: A representative lot detected nuclear Sox2 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human embryonic stem cells (hESCs) by fluorescent immunocytochemistry (Kingham, E., et al. (2013). Small. 9(12):2140-2151).
Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed iPSCs generated from human kidney tubular renal epithelial cells (Montserrat, N., et al. (2012). J. Biol. Chem. 287(29):24131-24138).
Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized iPSCs generated from the skin cells of patients with Parkinson′s disease (Sánchez-Danés, A., et al. (2012). EMBO Mol. Med. 4(5):380-395).
Immunocytochemistry Analysis: Representative lots detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized iPSCs generated from mouse ear cells and MEF (Burns, J.C., et al. (2012). PLoS One. 7(10):e48704; Hadadeh, O., et al. (2012). PLoS One. 7(11):e49065).
Western Blotting Analysis: A representative lot detected Sox2 in mouse embryonic stem cell (mESC) nuclear extracts (Banaszynski, L.A., et al. (2013). Cell. 155(1):107-120).
Immunofluorescence Analysis: A representative lot detected Sox2-positive cells in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized mouse brain vibratome sections by fluorescent immunohistochemistry (Matsuda, S., et al. (2012). J. Neurosci. 32(36):12543-12557).
Immunohistochemistry Analysis: A representative lot detected Sox2 immunoreactivity in 4% paraformaldehyde-fixed whole mount mouse embryo sections (van Rooijen, C., et al. Development. 139(14):2576-2583).

Minőség

Evaluated by Western Blotting in mouse embryonic stem cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SOX2 in 10 µg of mouse embryonic stem cell lysate.

Cél megnevezése

~39 kDa observed. 34.31 kDa (human) and 34.45 (mouse) calculated. Uncharacterized band(s) may appear in some lysates

Kapcsolódás

Replaces: AB5770

Analízis megjegyzés

Control
Immunoblot: Mouse or human embryonic stem cell lysate, mouse embryonic germ cell lysate
Immunocytochemistry: Human embryonic stem cells (H9 line)

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

The Sox2 high mobility group transcription factor inhibits mature osteoblast function in transgenic mice.
Holmes, G; Bromage, TG; Basilico, C
Bone null
Astrocytes derived from fetal neural progenitor cells as a novel source for therapeutic adenosine delivery.
Van Dycke, A; Raedt, R; Verstraete, A; Theofilas, P; Wadman, W; Vonck, K; Boison, D; Boon, P
Seizure : the Journal of the British Epilepsy Association null
A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics.
Muller, T; Fleischmann, G; Eildermann, K; Matz-Rensing, K; Horn, PA; Sasaki, E; Behr, R
Human Reproduction null
Stage-dependent modes of Pax6-Sox2 epistasis regulate lens development and eye morphogenesis.
Smith, AN; Miller, LA; Radice, G; Ashery-Padan, R; Lang, RA
Development null
Harish Babu et al.
Frontiers in neuroscience, 5, 89-89 (2011-08-04)
In vitro assays are valuable tools to study the characteristics of adult neural precursor cells under controlled conditions with a defined set of parameters. We here present a detailed protocol based on our previous original publication (Babu et al., 2007)
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Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

Protocols

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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