Ugrás a tartalomra
Merck
Összes fotó(1)

Key Documents

GF446

Sigma-Aldrich

Thermostable bFGF,Human

HumanKine®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture

Szinonimák:

FGF2, prostatropin, Prostatropin, HBGF-2, BFGF

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

UNSPSC kód:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

HumanKine® Thermostable bFGF,
Human Recombinant, Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.

Minőségi szint

100

Teszt

>95% (SDS-PAGE)

gyártó/kereskedő neve

HumanKine®

szennyeződések

<0.5 μg/mg protein endotoxin (<5 EU/μg)

UniProt elérési szám

P09038

Általános leírás

Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
The HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.

Alkalmazás

Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
HumanKine products are Xeno-Free
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors

Minőség

Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. EC50 is typically ≤0.5 ng/mL.

Fizikai forma

Lyophilized from a 0.2 μm filtered solution of 1X PBS.

Tárolás és stabilitás

Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.

Jogi információk

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Tárolási osztály kódja

13 - Non Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Qisheng Zuo et al.
EMBO reports, 23(1), e52491-e52491 (2021-11-09)
The unique characteristics of chicken primordial germ cells (PGCs) provide potential strategies for transgenic animal generation; however, insufficient PGC availability has limited their application. Regulation of bone morphogenic protein 4 (BMP4), a crucial factor for PGCs formation, may provide new
Fei Qian et al.
Molecular carcinogenesis, 58(4), 500-510 (2018-11-21)
HMGB1-RAGE signaling plays an integral role in inflammation-driven carcinogenesis. In the present study, we showed that RAGE has direct association with K-Ras following HMGB1 exposure in colorectal cancer (CRC) cells. Immunofluorescence analysis revealed a significant co-localization between RAGE and K-Ras
Qisheng Zuo et al.
Journal of cell science, 134(3) (2021-01-15)
The unique developmental characteristics of chicken primordial germ cells (PGCs) enable them to be used in recovery of endangered bird species, gene editing and the generation of transgenic birds, but the limited number of PGCs greatly limits their application. Studies
Megan Bowers et al.
Cell stem cell, 27(1), 98-109 (2020-05-11)
Altered neural stem/progenitor cell (NSPC) activity and neurodevelopmental defects are linked to intellectual disability. However, it remains unclear whether altered metabolism, a key regulator of NSPC activity, disrupts human neurogenesis and potentially contributes to cognitive defects. We investigated links between

Cikkek

HumanKine® Thermostable bFGF

Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással