8.51209

D(+)-Biotin

Novabiochem^®

• Szinonimák: D(+)-Biotin, D-Biotin,Vitamin H, Hexahydro-2-oxothieno(3,4-d)-imidazole-4-pentanoic acid, Vitamin H
• Tapasztalati képlet (Hill-képlet): C₁₀H₁₆N₂O₃S
• CAS-szám: 58-85-5
• Molekulatömeg: 244.31
• MDL-szám: MFCD00005541
• UNSPSC kód: 41116133
• EC index szám: 200-399-3
• NACRES: NA.22

Tulajdonságok

• Minőségi szint: 200
• termékcsalád: Novabiochem^®
• form: solid
• hatékonyság: >2000 mg/kg LD₅₀, oral (Rat)
• reakcióalkalmasság: reaction type: Biotinylations
• gyártó/kereskedő neve: Novabiochem^®
• pH: 4.5 (0.1 g/L in H₂O)
• mp: 232-233 °C
• oldhatóság: 0.2 g/L
• térfogatsúly: 200‑500 kg/m³
• alkalmazás(ok): peptide synthesis
• tárolási hőmérséklet: 2-8°C
• InChI: 1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)
• Nemzetközi kémiai azonosító kulcs: YBJHBAHKTGYVGT-UHFFFAOYSA-N

Leírás

Általános leírás

D(+)-Biotin is the simplest reagent for the introduction of biotin to peptides, proteins and other biomolecules. In SPPS, it is best coupled using DIPCDI/Oxyma Pure. Biotin-labeled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.^,,,

Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis

Literature references:
^[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
^[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
^[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
^[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.

Alkalmazás

Some recent applications of D(+)-Biotin in SPPS:

• In the high-throughput biotinylation of proteins. The approach for site-specific biotinylation of proteins is useful for various applications such as protein purification and analysis.
• In the site-specific biotinylation of multipodal glycoclusters on a solid support. The synthesis of biotinylated glycoclusters, has important applications in glycobiology.

Jogi információk

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable