616371

Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free

recombinant, expressed in Nicotiana, heat and acid-stable competitive, reversible inhibitor of serine proteases

• Szinonimák: Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free, Pancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor, Kallikrein-Trypsin Inactivator, Antikrein, Basic Pancreatic Trypsin Inhibitor
• Tapasztalati képlet (Hill-képlet): C₂₈₄H₄₃₂N₈₄O₇₉S₇
• CAS-szám: 9087-70-1
• Molekulatömeg: 6511.44
• MDL-szám: MFCD00130541
• UNSPSC kód: 12352202
• EC index szám: 2329949
• NACRES: NA.77

Tulajdonságok

• rekombináns: expressed in Nicotiana
• Minőségi szint: 200
• Teszt: ≥98% (SDS-PAGE)
• form: powder
• specifikus aktivitás: ≥5.0 units/mg protein
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze
• szín: white to off-white
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Recombinant, bovine aprotinin supplied without animal-derived components. Aprotinin is a competitive, reversible inhibitor of proteolytic and esterolytic activity. A relatively heat- and acid-stable serine protease inhibitor. Forms a tight complex, blocking the active site of the enzyme. Effective at concentrations equimolar with protease. Inhibits several proteases, including coagulation factors in the prephase of blood clotting, tissue and leukocytic proteinases, chymotrypsin, trypsin (K_d = 5 x 10^-14 M), plasmin (K_d = 2.3 x 10^-10 M), and kallikrein (K_d = 1 x 10^-7 M). Proteases not inhibited by aprotinin include Factor Xa, thrombin, pepsin, papain, and carboxypeptidases A and B. Useful for protein purification and for extending the life of cells in culture by preventing proteolytic damage.

Biokémiai/fiziológiai hatások

Cell permeable: no

Kd = 5 x 10-14 M, 2.3 x 10-10 M, 1 x 10-7 M against trypsin, plasmin, and kallikrein, respectively

Primary Target
chymotrypsin

Product does not compete with ATP.

Reversible: yes

Kiszerelés

Please refer to vial label for lot-specific concentration.

Figyelmeztetés

Toxicity: Standard Handling (A)

Egység definíció

One TIU (Trypsin Inhibitory Unit) will decrease the activity of two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 µmole of Nα-benzoyl-L-Arginine-p-Nitroanilide (L-BAPNA) per minute at pH 7.8 at 25°C.

Fizikai forma

Contains no animal-derived components.

Egyéb megjegyzések

Deutscher, M.P., 1990. Methods Enzymol.182, 83.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: nwg
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable