251765

DAR-4M AM

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• Szinonimák: DAR-4M AM, Diaminorhodamine-4M AM
• Tapasztalati képlet (Hill-képlet): C₂₈H₃₁IN₄O₅
• Molekulatömeg: 630.47
• UNSPSC kód: 12352202
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 100
• Teszt: ≥98% (HPLC)
• form: solution
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; protect from light
• szín: dark red
• fluoreszcencia: λ_ex ~560 nm; λ_em ~575 nm
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.

Biokémiai/fiziológiai hatások

Cell permeable: yes

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

Kiszerelés

Packaged under inert gas

Figyelmeztetés

Toxicity: Irritant (B)

Fizikai forma

In DMSO.

Feloldás

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

Egyéb megjegyzések

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Jogi információk

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Lobbanási pont (F): 188.6 °F
• Lobbanási pont (C): 87 °C