251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• Szinonimák: DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• Tapasztalati képlet (Hill-képlet): C₂₅H₁₈F₂N₂O₇
• CAS-szám: 254109-22-3
• Molekulatömeg: 496.42
• MDL-szám: MFCD16872050
• UNSPSC kód: 12352204
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 100
• Teszt: ≥98% (HPLC)
• form: solution
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; protect from light
• szín: pale yellow
• fluoreszcencia: λ_ex ~500 nm; λ_em ~515 nm
• tárolási hőmérséklet: −20°C
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• Nemzetközi kémiai azonosító kulcs: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Leírás

Általános leírás

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Biokémiai/fiziológiai hatások

Primary Target
Nitric oxide (NO) fluorescent indicator

Figyelmeztetés

Toxicity: Irritant (B)

Fizikai forma

A 5 mM solution in DMSO.

Feloldás

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

Egyéb megjegyzések

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

Jogi információk

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Lobbanási pont (F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Lobbanási pont (C): 87 °C - closed cup - (Dimethylsulfoxide)