220201

CHAPS

Molecular Biology Grade

• Szinonimák: CHAPS, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• Tapasztalati képlet (Hill-képlet): C₃₂H₅₈N₂O₇S
• CAS-szám: 75621-03-3
• Molekulatömeg: 614.88
• MDL-szám: MFCD00012116
• UNSPSC kód: 12161703
• NACRES: NA.25

Tulajdonságok

• Terméknév: CHAPS, Molecular Biology Grade,
• grade: Molecular Biology
• Minőségi szint: 300
• leírás: Merck USA index - 14, 2044; zwitterionic
• Teszt: ≥98% (NMR)
• Forma: crystalline powder
• molekulatömeg: micellar avg mol wt 6150
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; desiccated (hygroscopic)
• aggregált szám: 10
• szín: white
• CMC: 6 - 10 mM; 6 mM (20-25°C)
• mp: 157 °C (315 °F)
• átmeneti hőmérséklet: cloud point >100 °C
• oldhatóság: water: 1.0 M
• idegen aktivitás: DNase, RNase, and Protease, none detected
• kiszállítva: ambient
• tárolási hőmérséklet: 15-25°C
• SMILES string: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• Nemzetközi kémiai azonosító kulcs: UMCMPZBLKLEWAF-RFCNGIAKSA-N

Leírás

Általános leírás

Conductivity (1.0 M, 24°C): <100 µmhos.

Non-denaturing zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than the structurally related carboxylic acid anions, and is more effective at breaking protein-protein interactions than either sodium cholate or TRITON^™ X-100. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding to opiates. Also capable of disaggregating P 450 to its monomeric form without denaturation. Has a small micellar molecular weight (6150 Daltons) and a high critical micellar concentration (6-10 mM). Can be removed from either gels or protein solutions by dialysis. Aggregation number: 4-14.

Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON^™ X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14.

Alkalmazás

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Figyelmeztetés

Toxicity: Harmful (C)

Feloldás

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.

Egyéb megjegyzések

Chen, Y., et al. 1992. Biochemistry 31, 2415.
Ofri, D., et al. 1992. J. Neurochem. 58, 628.
Ransom, R.W., et al. 1992. Biochem. Pharmacol. 43, 1823.
Simonds, W.F., et al. 1992. Proc. Natl. Acad. Sci. USA77, 4623.
Yannariello-Brown, J., and Weigel, P.H. 1992. Biochemistry31, 576.
Hjelmeland, L.M. 1980. Proc. Natl. Acad. Sci. USA77, 6368.
Simonds, W.F., et al. 1980. Proc. Natl. Acad. Sci. USA77, 4623.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Biztonsági adatok

• Piktogramok: GHS07
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H302
• Óvintézkedésre vonatkozó mondatok: P264 - P270 - P301 + P312 - P501
• Veszélyességi osztályok: Acute Tox. 4 Oral
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable