19899

CHAPS 100 mM solution

• Szinonimák: CHAPS solution
• Tapasztalati képlet (Hill-képlet): C₃₂H₅₈N₂O₇S
• CAS-szám: 75621-03-3
• Molekulatömeg: 614.88
• MDL-szám: MFCD00012116
• UNSPSC kód: 12161902
• PubChem Substance ID: 57647807
• NACRES: NA.25

Tulajdonságok

• leírás: zwitterionic
• Minőségi szint: 100
• form: liquid
• molekulatömeg: 614.88 g/mol
• koncentráció: 100 mM in H₂O
• technika/technikák: protein quantification: suitable
• CMC: 8 mM
• átmeneti hőmérséklet: cloud point >100 °C
• sűrűség: 1.01 g/mL at 20 °C
• tárolási hőmérséklet: 2-8°C
• SMILES string: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• Nemzetközi kémiai azonosító kulcs: UMCMPZBLKLEWAF-RFCNGIAKSA-N

Leírás

Általános leírás

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.

Alkalmazás

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.

Biokémiai/fiziológiai hatások

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves