07-434

Anti-BRCA1 Antibody

serum, Upstate^®

• Szinonimák: Anti-BRCAI, Anti-BRCC1, Anti-BROVCA1, Anti-FANCS, Anti-IRIS, Anti-PNCA4, Anti-PPP1R53, Anti-PSCP, Anti-RNF53
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: serum
• antitest terméktípus: primary antibodies
• klón: polyclonal
• faj reaktivitás: human
• gyártó/kereskedő neve: Upstate^®
• technika/technikák: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotípus: IgG
• NCBI elérési szám: NM_007294
• UniProt elérési szám: P38398
• kiszállítva: dry ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... BRCA1(672)

Leírás

Általános leírás

Two major susceptibility genes for breast cancer, BRCA1 and BRCA2, have been identified. Both genes are considered to be tumor-suppressor genes. Mutations within BRCA1 and BRCA2 are responsible for most familial breast cancer cases. Functional analyses of the BRCA1 and BRCA2 gene products have established their dual participation in transcription regulation and DNA damage repair. BRCA1 affects cell cycle regulation and loss of BRCA1 function due to decreased expression leads to cell cycle arrest, through p53 and p21 genes. In vivo, BRCA1 and BRCA2 are expressed at maximal levels in rapidly proliferating cells. This feature is consistent with in vitro observations that BRCA1 and BRCA2 are expressed in a cell cycle-dependent manner. During mammary gland development, the expression of BRCA1 and BRCA2 is induced in rapidly proliferating cellular compartments.

Egyediség

Recognizes human BRCA1.

Immunogén

GST-tagged fusion protein corresponding to amino acids 1301-1863 of human BRCA1.

Alkalmazás

Detect BRCA1 with Anti-BRCA1 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC, IP & WB. This BRCA1 antibody has been tested in human samples.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Minőség

routinely evaluated by immunoblot in HeLa nuclear extract

Cél megnevezése

220 kDa

Fizikai forma

Serum containing 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

Unpurified

Tárolás és stabilitás

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analízis megjegyzés

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable