07-274
Anti-dimethyl-Histone H3 (Lys36) Antibody
serum, Upstate®
Szinonimák:
H3K36me2, Histone H3 (di methyl K36), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ChIP
DB
WB
DB
WB
faj reaktivitás:
mouse, Drosophila, Schizosaccharomyces pombe, Saccharomyces cerevisiae, vertebrates, human, chicken
technika/technikák:
ChIP: suitable
dot blot: suitable
western blot: suitable
dot blot: suitable
western blot: suitable
citations:
49
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
serum
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
mouse, Drosophila, Schizosaccharomyces pombe, Saccharomyces cerevisiae, vertebrates, human, chicken
gyártó/kereskedő neve
Upstate®
technika/technikák
ChIP: suitable
dot blot: suitable
western blot: suitable
izotípus
IgG
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
dimethylation (Lys36)
Géninformáció
human ... H3C1(8350)
mouse ... H3C1(360198)
Általános leírás
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Egyediség
A broad species cross-reactivity is expected.
Recognizes methylated Histone H3 (Lys36), Mr 17 kDa as determined by Immunoblotting. Does not cross react with peptides containing dimethyl-histone H4 Lys20, nor Histone H3 Lys4, Lys9, or Lys27.
Immunogén
Epitope: Lys36
KLH-conjugated, synthetic peptide corresponding to amino acids 30-38 (CPATGGV(dimethyl-K)KP) of Histone H3 with a N-terminal C added for conjugation. The immunizing sequence is conserved from Tetrahymena to human.
Alkalmazás
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-dimethyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect dimethyl-Histone H3 (Lys36) also known as H3K36me2, Histone H3 (di methyl K36).
Minőség
Routinely evaluated by western blot on in acid-extracted proteins from sodium butyrate treated cells.
Western Blot Analysis:
A 1:1000 dilution of this lot detected methylated Histone H3 in acid-extracted proteins from untreated HeLa cells (Catalog # 13-112). The antibody did not detect recombinant Xenopus laevis histone H3 purified from E. coli (Catalog # 14-411).
Western Blot Analysis:
A 1:1000 dilution of this lot detected methylated Histone H3 in acid-extracted proteins from untreated HeLa cells (Catalog # 13-112). The antibody did not detect recombinant Xenopus laevis histone H3 purified from E. coli (Catalog # 14-411).
Cél megnevezése
17 kDa
Fizikai forma
Rabbit polyclonal IgG immunodepleted antiserum in buffer containing 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC. Serum was passed over immunoaffinity column containing the non-modified peptide.
Unpurified
Tárolás és stabilitás
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analízis megjegyzés
Control
Sodium butyrate treated, acid extracted HeLa cell lysate.
Sodium butyrate treated, acid extracted HeLa cell lysate.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Succinate inhibition of alpha-ketoglutarate-dependent enzymes in a yeast model of paraganglioma.
Smith, EH; Janknecht, R; Maher, LJ
Human Molecular Genetics null
Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression
Strahl, B. D., et al
Molecular and cellular biology, 22, 1298-1306 (2002)
Hai-Ning Du et al.
The Journal of biological chemistry, 285(15), 11704-11713 (2010-02-09)
Set2-mediated H3 Lys(36) methylation is a histone modification that has been demonstrated to function in transcriptional elongation by recruiting the Rpd3S histone deacetylase complex to repress intragenic cryptic transcription. Recently, we identified a trans-histone pathway in which the interaction between
Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development.
Sun, Qianwen and Zhou, Dao-Xiu
Proceedings of the National Academy of Sciences of the USA, 105, 13679-13684 (2008)
Robert Jeffrey Schmitz et al.
Plant physiology, 149(2), 1196-1204 (2008-12-19)
The spectrum of histone modifications at a given locus is a critical determinant for the correct output of gene expression. In Arabidopsis (Arabidopsis thaliana), many studies have examined the relationship between histone methylation and gene expression, but few studies exist
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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