803219

Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

• Szinonimák: Ethylene glycolbis(sulfosuccinimidylsuccinate), bis(sulfosuccinimidyl) 4,4′-[(ethylene)dioxy]bis(4-oxobutanoate)
• Tapasztalati képlet (Hill-képlet): C₁₈H₁₈O₁₈N₂Na₂S₂
• CAS-szám: 167410-92-6
• Molekulatömeg: 660.45
• MDL-szám: MFCD00054988
• UNSPSC kód: 12352200
• PubChem Substance ID: 329768789
• NACRES: NA.22

Tulajdonságok

• form: powder
• Minőségi szint: 100
• molekulatömeg: 660.45
• reakcióalkalmasság: reagent type: cross-linking reagent
• tárolási körülmény: desiccated
• oldhatóság: water: soluble
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C
• InChI: 1S/C18H20N2O18S2.2Na/c21-11-7-9(39(29,30)31)17(27)19(11)37-15(25)3-1-13(23)35-5-6-36-14(24)2-4-16(26)38-20-12(22)8-10(18(20)28)40(32,33)34;;/h9-10H,1-8H2,(H,29,30,31)(H,32,33,34);;/q;2*+1/p-2
• Nemzetközi kémiai azonosító kulcs: IYBKWXQWKPSYDT-UHFFFAOYSA-L

Leírás

Általános leírás

EGS is a water-insoluble, homobifunctional N-hydroxysuccinimide ester (NHS ester) and Sulfo-EGS is its water-soluble analog. The spacer arm contains two cleavable ester sites that may be broken with hydroxylamine, which yields two fragments with terminal amide bonds and the release of ethylene glycol. These reagents are often used for conjugating radiolabeled ligands to cell surface receptors. The water insoluble reagent EGS does not possess a charged group, is lipophilic and, therefore, membrane-permeable and useful for intracellular and intramembrane protein conjugation. EGS is first dissolved in DMSO or DMF and added to the aqueous reaction mixture at a final solvent concentration of 10-20%, to minimize detrimental affects to the protein.

Tulajdonságok és előnyök

• Reactive groups: sulfo-NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Water-soluble; compare to EGS
• Membrane-impermeable, allowing for cell surface labeling
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Vigyázat

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Biztonsági adatok

• Piktogramok: GHS07
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H315,H319,H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable