Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Journal of agricultural and food chemistry (2004-08-19)

Dawn M Chapman, John H Thorngate, Mark A Matthews, Jean-Xavier Guinard, Susan E Ebeler

RESUMO

A rapid and automated solid phase microextraction (SPME) stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for 2-methoxy-3-isobutylpyrazine (MIBP) quantification in red wine was developed. Wines with 30% (w/v) NaCl and 2-methoxy-(2)H(3)-3-isobutylpyrazine internal standard were sampled with a 2 cm divinylbenzene/carboxen/poly(dimethylsiloxane) SPME fiber for 30 min at 40 degrees C and analyzed by GC-MS. The method was used to measure MIBP concentrations in Cabernet Sauvignon wines that were produced from six winter pruning treatments over two vintages. MIBP concentrations were significantly negatively correlated with buds per vine. In addition, the MIBP concentration was directly related to sensory vegetal intensity ratings obtained by descriptive analysis.

MATERIAIS

Número do produto

Marca

Descrição do produto

57348-U

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Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), needle size 24 ga, StableFlex

57328-U

Supelco

Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder

57298-U

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Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler

57299-U

Supelco

Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm

57329-U

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Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler

20358

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ORBO^™ 90 Carboxen^® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

10478-U

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Adsorvente Carboxen^®, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

20355

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ORBO^™ 78 HBr on Carboxen^® 564 specially cleaned (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

25467

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Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57318

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Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), d_f 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

57295-U

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Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler

25461

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Coluna de GC capilar Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57319

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Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler

57335-U

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Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler

24246

Supelco

Carboxen^®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

W313203

Sigma-Aldrich

2-Isobutyl-3-methoxypyrazine, ≥99%, FG

57334-U

Supelco

Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber

57343-U

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Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler

47528-U

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2-Isobutyl-3-methoxypyrazine solution, certified reference material

57344-U

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Conjunto de fibras de SPME Carboxeno/polidimetilsiloxano (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder

11072-U

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Adsorvente Carboxen^®, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

297666

Sigma-Aldrich

2-Isobutyl-3-methoxypyrazine, 99%

24241-U

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Coluna de GC capilar Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

28718-U

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Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

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Adsorvente Carboxen^®, matrix Carboxen^® 1000, 40-60 mesh, bottle of 50 g

10269

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57914-U

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10471

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ORBO^™-93 Sorbent Tube, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea