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T3449

Sigma-Aldrich

TrypZean® Solution, 1×

recombinant, expressed in corn, sterile-filtered

Sinônimo(s):

Trypsin

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100 ML
R$ 324,00
500 ML
R$ 1.053,00

R$ 324,00


Previsão de entrega em27 de março de 2025


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100 ML
R$ 324,00
500 ML
R$ 1.053,00

About This Item

Código UNSPSC:
12352204
NACRES:
NA.75

R$ 324,00


Previsão de entrega em27 de março de 2025


Solicite uma grande encomenda

recombinante

expressed in corn

Nível de qualidade

esterilidade

sterile-filtered

Formulário

aqueous solution

concentração

1 ×

técnica(s)

cell culture | mammalian: suitable

pH

7.70-8.30

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.

Aplicação

TrypZean® Solution, 1× has been used:

  • for the suspension of pellet during DNA extraction[1]
  • digestion of cells to single-cell suspension for pharmacotyping of 2D cells[2]
  • as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium[3]

Características e benefícios

TrypZean® Solution provides the following features and benefits:
  • Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
  • TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
  • Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
  • High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
  • Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines

Informações legais

Trypzean is a registered trademark of ProdiGene, Inc.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Samia Rourou et al.
Biotechnology progress, 25(6), 1752-1761 (2009-09-22)
This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Katja Peschke et al.
EMBO molecular medicine, 14(4), e14876-e14876 (2022-02-05)
Despite the advance and success of precision oncology in gastrointestinal cancers, the frequency of molecular-informed therapy decisions in pancreatic ductal adenocarcinoma (PDAC) is currently neglectable. We present a longitudinal precision oncology platform based on functional model systems, including patient-derived organoids
Clay O Lacefield et al.
Cell reports, 26(8), 2000-2008 (2019-02-21)
The mammalian brain can form associations between behaviorally relevant stimuli in an animal's environment. While such learning is thought to primarily involve high-order association cortex, even primary sensory areas receive long-range connections carrying information that could contribute to high-level representations.

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Questions

  1. What is the working concentration of recombinant trypsin used ?

    1 answer
    1. Product T3449 is prepared from a 0.0025% concentration.

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