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C5914

Sigma-Aldrich

Cell Dissociation Solution Non-enzymatic 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

Sinônimo(s):

Dissociation Solution

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100 ML
R$ 270,00

R$ 270,00


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100 ML
R$ 270,00

About This Item

Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.75

R$ 270,00


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Nível de qualidade

esterilidade

sterile-filtered

linha de produto

BioReagent

Formulário

liquid

concentração

1 ×

técnica(s)

cell culture | mammalian: suitable

pH

6.9-7.1

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

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Descrição geral

Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.[1]

Aplicação

Cell Dissociation Solution Non-enzymatic 1x has been used for the dissociation of retinal astrocytes culture,[2] U87 glioblastoma tumor cells[3] and cardiac stem cells (CSCs).[4]
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Questions

1–5 of 5 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    1 answer
    1. We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

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  3. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    1 answer
    1. It will work with most cell types.

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  4. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    1 answer
    1. Yes, it does contain EDTA; the concentration is proprietary.

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  5. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    1 answer
    1. No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

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