T2815

Transforming Growth Factor-β2 human

≥97% (electrophoresis), recombinant, expressed in NSO cells, powder, suitable for cell culture

• Sinônimo(s): hTGF-β2, TGF-β2
• Número MDL: MFCD00163957
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Nome do produto: Transforming Growth Factor-β2 human, TGF-β2, recombinant, expressed in NSO cells, powder, suitable for cell culture
• fonte biológica: human
• Nível de qualidade: 200
• recombinante: expressed in NSO cells
• Ensaio: ≥97% (electrophoresis)
• Formulário: powder
• potência: 0.04 ng/mL ED₅₀
• qualidade: endotoxin tested
• peso molecular: 25 kDa
• embalagem: pkg of 10 μg; pkg of 2 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• técnica(s): cell culture | mammalian: suitable
• Impurezas: ≤1 EU/μg
• adequação: suitable for
• nº de adesão UniProt: P61812
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... TGFB2(7042) , TGFBR2(7048)

Descrição

Descrição geral

The transforming growth factor-β2 (TGFB2) gene is mapped to human chromosome 1q41.[1] The encoded protein is localized to extracellular matrix.[2] TGF-β2 is a member of the TGF-β superfamily.[3] TGF-β exists in three isoforms (TGF-β1, TGF-β2 and TGF-β3) and are known to share similar structure and function.[4]

Aplicação

Transforming Growth Factor-β2 human has been used in chondrogenic, adipogenic and osteogenic differentiation of mesenchymal stem cells.[5] It has also been used to induce extracellular matrix accumulation in primary human trabecular meshwork cells and further to study the action of tetramethylpyrazine on the same.[6]

Ações bioquímicas/fisiológicas

TGF-β2 is known to stimulate cell growth, differentiation, migration and survival.[7] TGF-β2 acts as a key regulator for immune homeostasis by controlling lymphocyte proliferation, embryogenesis, hematopoiesis and apoptosis.[6] TGF-β is also associated with inflammation and wound healing and is considered as a strong growth inhibitor for keratinocyte cells.[8][9] TGF-β is associated with the process of tumor development, progression and metastasis.[10] TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis.[7] The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions.[4]

Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

forma física

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.1 mg bovine serum albumin

Nota de análise

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)