10109886001

Trypsin Inhibitor

from soybean

• Sinônimo(s): trypsin
• Código UNSPSC: 12352207

Propriedades

• fonte biológica: soybean
• Nível de qualidade: 100
• forma: lyophilized
• peso molecular: M_r 20,100
• embalagem: pkg of 50 mg
• fabricante/nome comercial: Roche
• concentração: 1 mg/mL
• técnica(s): cell culture | mammalian: suitable
• pH: 3.8-4.5
• solubilidade: water: soluble
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.

Especificidade

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).

Aplicação

Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.

Trypsin Inhibitor has been used:

• in the buffer for hepatocyte isolation
• to inhibit trypsin in Corneal epithelial cells
• to inhibit papain activity while treating hippocampal neural stem cells (NSCs)

Ações bioquímicas/fisiológicas

Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.

Especificações

Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)

Isoelectric point: 4.5

Definição da unidade

1 U = 270 S & T-units or 90 USP-units

Nota de preparo

Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.

Reconstituição

Sensitive to heat, alkaline pH, and protein-precipitating compounds.

Armazenamento e estabilidade

Store at 2 to 8 °C. (Store dry!)

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable