11412272001

Transforming Growth Factor-β1, human (hTGF-β1)

recombinant (CHO cells)

• Sinônimo(s): hTGF
• Código UNSPSC: 12352207

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• recombinante: expressed in CHO cells
• Ensaio: >95% (SDS-PAGE)
• Formulário: solution
• potência: <0.1 ng/mL EC₅₀
• peso molecular: 25,000 Da
• embalagem: pkg of 1 mL (1 μg)
• fabricante/nome comercial: Roche
• condição de armazenamento: avoid repeated freeze/thaw cycles
• Impurezas: <0.1 EU/μgtested (LAL test)
• nº de adesão UniProt: P01137
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... TGFB1(7040)

Descrição

Descrição geral

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

Especificidade

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC₅₀: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

Aplicação

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.[1]

Ações bioquímicas/fisiológicas

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.[2]

Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.[3]

Qualidade

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

Sequência

Chain Length 2 x 112 AA

Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

Definição da unidade

EC₅₀ definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

Nota de preparo

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash