H8541

Fator de crescimento transformador-β1

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Sinônimo(s): TGF-β1
• Número MDL: MFCD00166089
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Nome do produto: Fator de crescimento transformador-β1, TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture
• fonte biológica: human
• Nível de qualidade: 300
• recombinante: expressed in HEK 293 cells
• Ensaio: ≥95% (SDS-PAGE)
• Formulário: lyophilized powder
• potência: ≤0.5 ng/mL EC₅₀
• qualidade: endotoxin tested
• peso molecular: dimer 25 kDa (non-glycosylated)
• embalagem: pkg of 5 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• técnica(s): cell culture | mammalian: suitable
• Impurezas: ≤1 EU/μg
• nº de adesão UniProt: P01137
• temperatura de armazenamento: -10 to -25°C
• Informações sobre genes: human ... TGFB1(7040)

Descrição

Descrição geral

The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide.[1][2] TGF-ß1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer.[3]

Aplicação

Transforming Growth Factor-β1 (TGF-β) human has been used :

• to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling.[4]
• to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device.[5]
• to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes.[6]

Ações bioquímicas/fisiológicas

O fator de crescimento transformador β1 (TGF-β1) é produzido em diversos tipos de células, mas consta que é mais concentrado em plaquetas de mamíferos, onde está presente em níveis cerca de quatro vezes maiores que os de TGF-β2.

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types.[2] Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function.[7] TGF-β1 is associated with the developmental and metastasis of colorectal cancer.[8]

forma física

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Nota de análise

The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Danger
• Frases de perigo: H314
• Declarações de precaução: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Classificações de perigo: Eye Dam. 1 - Skin Corr. 1B
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable